Effect of luminal vasopressin on NaCl transport in the medullary thick ascending limb of the rat

Jay Grider; Jeff Falcone; Eric Kilpatrick; Cobern Ott; Brian Jackson

doi:10.1016/0014-2999(96)00620-6

Effect of luminal vasopressin on NaCl transport in the medullary thick ascending limb of the rat

Jay Grider, Jeff Falcone, Eric Kilpatrick, Cobern Ott, Brian Jackson

Physiology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The aim of the present study was to determine whether vasopressin affects NaCl reabsorption in the medullary thick ascending limb of the loop of Henle when administered selectively to the luminal membrane. At 5 x 10^-6 M and 10^-8 M, luminal [Arg⁸]vasopressin significantly inhibited Cl^- transport in the in vitro microperfused rat medullary thick ascending limb by 46.4 ± 5.9% (P < 0.01) and 32.4 ± 2.0% (P < 0.05) respectively. The response to 10^-8 M luminal [Arg⁸]vasopressin was completely blocked by the vasopressin V₁ receptor antagonist [β-mercapto-β,β-cyclopenta-methylenepropionyl¹,O-Me-Tyr²,Arg⁸]vasopressin (10^-6 M), and was mimicked by the vasopressin V₁ receptor agonist [Phe¹,Ile⁵,Orn⁸]vasopressin (10^-8 M; Δ -35.0 ± 4.5%; P < 0.05). Luminal administration of the vasopressin V₂ receptor agonist [deamino-Cys¹,D-Arg⁸]vasopressin (5 x 10^-6 M) had no effect on transport. These data suggest that luminal vasopressin can inhibit NaCl transport in the medullary thick ascending limb of the rat via vasopressin V₁ receptors.

Original language	English
Pages (from-to)	115-118
Number of pages	4
Journal	European Journal of Pharmacology
Volume	313
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-2999(96)00620-6
State	Published - Oct 10 1996

Bibliographical note

Funding Information:
These studies were supported by grants from the American Heart Association, Kentucky Affiliate (B.A.J.), NIH grant HL 538 18 (J.C.F.) and NASA EPSCoR WKU 522611 (C.E.O.).

Keywords

Ion transport
Loop of Henle
Microperfusion, in vitro
[Arg]Vasopressin

ASJC Scopus subject areas

Pharmacology

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10.1016/0014-2999(96)00620-6

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title = "Effect of luminal vasopressin on NaCl transport in the medullary thick ascending limb of the rat",

abstract = "The aim of the present study was to determine whether vasopressin affects NaCl reabsorption in the medullary thick ascending limb of the loop of Henle when administered selectively to the luminal membrane. At 5 x 10-6 M and 10-8 M, luminal [Arg8]vasopressin significantly inhibited Cl- transport in the in vitro microperfused rat medullary thick ascending limb by 46.4 ± 5.9% (P < 0.01) and 32.4 ± 2.0% (P < 0.05) respectively. The response to 10-8 M luminal [Arg8]vasopressin was completely blocked by the vasopressin V1 receptor antagonist [β-mercapto-β,β-cyclopenta-methylenepropionyl1,O-Me-Tyr2,Arg8]vasopressin (10-6 M), and was mimicked by the vasopressin V1 receptor agonist [Phe1,Ile5,Orn8]vasopressin (10-8 M; Δ -35.0 ± 4.5%; P < 0.05). Luminal administration of the vasopressin V2 receptor agonist [deamino-Cys1,D-Arg8]vasopressin (5 x 10-6 M) had no effect on transport. These data suggest that luminal vasopressin can inhibit NaCl transport in the medullary thick ascending limb of the rat via vasopressin V1 receptors.",

keywords = "Ion transport, Loop of Henle, Microperfusion, in vitro, [Arg]Vasopressin",

author = "Jay Grider and Jeff Falcone and Eric Kilpatrick and Cobern Ott and Brian Jackson",

note = "Funding Information: These studies were supported by grants from the American Heart Association, Kentucky Affiliate (B.A.J.), NIH grant HL 538 18 (J.C.F.) and NASA EPSCoR WKU 522611 (C.E.O.).",

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doi = "10.1016/0014-2999(96)00620-6",

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AU - Grider, Jay

AU - Falcone, Jeff

AU - Kilpatrick, Eric

AU - Ott, Cobern

AU - Jackson, Brian

N1 - Funding Information: These studies were supported by grants from the American Heart Association, Kentucky Affiliate (B.A.J.), NIH grant HL 538 18 (J.C.F.) and NASA EPSCoR WKU 522611 (C.E.O.).

PY - 1996/10/10

Y1 - 1996/10/10

N2 - The aim of the present study was to determine whether vasopressin affects NaCl reabsorption in the medullary thick ascending limb of the loop of Henle when administered selectively to the luminal membrane. At 5 x 10-6 M and 10-8 M, luminal [Arg8]vasopressin significantly inhibited Cl- transport in the in vitro microperfused rat medullary thick ascending limb by 46.4 ± 5.9% (P < 0.01) and 32.4 ± 2.0% (P < 0.05) respectively. The response to 10-8 M luminal [Arg8]vasopressin was completely blocked by the vasopressin V1 receptor antagonist [β-mercapto-β,β-cyclopenta-methylenepropionyl1,O-Me-Tyr2,Arg8]vasopressin (10-6 M), and was mimicked by the vasopressin V1 receptor agonist [Phe1,Ile5,Orn8]vasopressin (10-8 M; Δ -35.0 ± 4.5%; P < 0.05). Luminal administration of the vasopressin V2 receptor agonist [deamino-Cys1,D-Arg8]vasopressin (5 x 10-6 M) had no effect on transport. These data suggest that luminal vasopressin can inhibit NaCl transport in the medullary thick ascending limb of the rat via vasopressin V1 receptors.

AB - The aim of the present study was to determine whether vasopressin affects NaCl reabsorption in the medullary thick ascending limb of the loop of Henle when administered selectively to the luminal membrane. At 5 x 10-6 M and 10-8 M, luminal [Arg8]vasopressin significantly inhibited Cl- transport in the in vitro microperfused rat medullary thick ascending limb by 46.4 ± 5.9% (P < 0.01) and 32.4 ± 2.0% (P < 0.05) respectively. The response to 10-8 M luminal [Arg8]vasopressin was completely blocked by the vasopressin V1 receptor antagonist [β-mercapto-β,β-cyclopenta-methylenepropionyl1,O-Me-Tyr2,Arg8]vasopressin (10-6 M), and was mimicked by the vasopressin V1 receptor agonist [Phe1,Ile5,Orn8]vasopressin (10-8 M; Δ -35.0 ± 4.5%; P < 0.05). Luminal administration of the vasopressin V2 receptor agonist [deamino-Cys1,D-Arg8]vasopressin (5 x 10-6 M) had no effect on transport. These data suggest that luminal vasopressin can inhibit NaCl transport in the medullary thick ascending limb of the rat via vasopressin V1 receptors.

KW - Ion transport

KW - Loop of Henle

KW - Microperfusion, in vitro

KW - [Arg]Vasopressin

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JF - European Journal of Pharmacology

IS - 1-2

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Effect of luminal vasopressin on NaCl transport in the medullary thick ascending limb of the rat

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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