TY - JOUR
T1 - Effect of major nutrients on podophyllotoxin production in Podophyllum hexandrum suspension cultures
AU - Chattopadhyay, S.
AU - Mehra, R. S.
AU - Srivastava, A. K.
AU - Bhojwani, S. S.
AU - Bisaria, V. S.
PY - 2003/1
Y1 - 2003/1
N2 - The effect of major medium ingredients (sugar, nitrogen source and phosphate) in Podophyllum hexandrum suspension cultures was investigated in order to increase the production of podophyllotoxin, the raw material in the synthesis of anticancer drugs. Amongst B5, Eriksson, MS, Nitsch, Street and White's medium, MS medium resulted in high growth and podophyllotoxin accumulation. The optimum level of nitrogen was found to be 60 mM, with a combination of ammonium salts and nitrate in the ratio of 1:2. The highest level of podophyllotoxin was obtained at 60 g glucose/1 and at 1.25 mM phosphate after 30 days. Statistical design was adopted to determine the optimum levels of the parameters for cell growth and podophyllotoxin production.
AB - The effect of major medium ingredients (sugar, nitrogen source and phosphate) in Podophyllum hexandrum suspension cultures was investigated in order to increase the production of podophyllotoxin, the raw material in the synthesis of anticancer drugs. Amongst B5, Eriksson, MS, Nitsch, Street and White's medium, MS medium resulted in high growth and podophyllotoxin accumulation. The optimum level of nitrogen was found to be 60 mM, with a combination of ammonium salts and nitrate in the ratio of 1:2. The highest level of podophyllotoxin was obtained at 60 g glucose/1 and at 1.25 mM phosphate after 30 days. Statistical design was adopted to determine the optimum levels of the parameters for cell growth and podophyllotoxin production.
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U2 - 10.1007/s00253-002-1149-7
DO - 10.1007/s00253-002-1149-7
M3 - Article
C2 - 12536253
AN - SCOPUS:0037255507
SN - 0175-7598
VL - 60
SP - 541
EP - 546
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 5
ER -