Effect of mifepristone on pregnancy, pregnancy-specific protein B (PSPB), progesterone, estradiol-17β, prostaglandin F2α (PGF2α) and prostaglandin E (PGE) in ovariectomized 90-day pregnant ewes

Y. S. Weems; P. J. Bridges; R. G. Sasser; L. Ching; B. R. LeaMaster; D. L. Vincent; C. W. Weems

doi:10.1016/S0090-6980(02)00110-7

Effect of mifepristone on pregnancy, pregnancy-specific protein B (PSPB), progesterone, estradiol-17β, prostaglandin F_2α (PGF_2α) and prostaglandin E (PGE) in ovariectomized 90-day pregnant ewes

Y. S. Weems, P. J. Bridges, R. G. Sasser, L. Ching, B. R. LeaMaster, D. L. Vincent, C. W. Weems

Animal and Food Sciences

Research output: Contribution to journal › Article › peer-review

13 Citations (SciVal)

Abstract

The objective of this experiment was to determine the effect of mifepristone, a progesterone receptor antagonist, on pregnancy and secretion of steroids, pregnancy-specific protein B (PSPB) and prostaglandins at mid-pregnancy in ewes. Ninety-day pregnant ewes were ovariectomized (OVX) and treatments were initiated 72h post-OVX. Ewes received (1) vehicle, (2) prostaglandin F_2α (PGF_2α, 8mg/58kg/bw, i.m.) 84h post-OVX, (3) mifepristone (50mg intrajugular at 72, 84, 96, and 108h post-OVX), (4) mifepristone (50mg)+PGF_2α, (5) mifepristone (100mg intrajugular at 72, 84, 96, and 108h), and (6) mifepristone (100mg)+PGF_2α. Ewes treated with vehicle or PGF_2α alone did not abort (P≥0.05). But, 60, 80, 60, and 100% of ewes treated with mifepristone (50mg), mifepristone (50mg)+PGF_2α, mifepristone (100mg), and mifepristone (100mg)+PGF_2α, respectively, aborted (P≤0.05). Profiles of progesterone, estradiol-17β, prostaglandin E (PGE), or PSPB did not differ (P≥0.05) among treatment groups. Profiles of PGF_2α of treatment groups receiving mifepristone with or without PGF_2α differed (P<0.05) from vehicle or PGF_2α alone-treated ewes. It is concluded that progesterone actions are necessary to suppress uterine/placental secretion of PGF_2α and that maintenance of critical progesterone: estradiol-17β and PGE:PGF_2α ratios are necessary for maintenance of pregnancy.

Original language	English
Pages (from-to)	195-208
Number of pages	14
Journal	Prostaglandins and Other Lipid Mediators
Volume	70
Issue number	1-2
DOIs	https://doi.org/10.1016/S0090-6980(02)00110-7
State	Published - Sep 2002

Bibliographical note

Funding Information:
The authors gratefully acknowledge the assistance of Mr. Alan Umaki and Mr. Armand Unabia, Waialee Livestock Research Farm for care, estrous detection, and mating of sheep used in these studies. The antiserum for the progesterone assay was kindly supplied by Dr. R.L. Butcher, West Virginia University; antiserum for PGF 2α was kindly provided by Dr. L. Levine, Brandeis University, Waltham, MA; and antiserum for PGE assays was kindly supplied by Dr. N. Mason, Lily Research Laboratories, Indianapolis, IN. Also the authors acknowledge the support of Mr. Bruce Robinson, Niihau Ranch. The authors also want to thank Mr. Eric Johnson, Hawaii Mega-Cor, Honolulu, HI for providing surgical supplies. The authors want to thank Mr. Wayne Toma for assistance with statistical analysis and computer graphics and Ms. Beverly Chang for typing of the manuscript.

Keywords

Ewes
Mifepristone
Pregnancy
Prostaglandin PGF

ASJC Scopus subject areas

Biochemistry
Physiology
Pharmacology
Cell Biology

Access to Document

10.1016/S0090-6980(02)00110-7

Cite this

Weems, Y. S., Bridges, P. J., Sasser, R. G., Ching, L., LeaMaster, B. R., Vincent, D. L., & Weems, C. W. (2002). Effect of mifepristone on pregnancy, pregnancy-specific protein B (PSPB), progesterone, estradiol-17β, prostaglandin F_2α (PGF_2α) and prostaglandin E (PGE) in ovariectomized 90-day pregnant ewes. Prostaglandins and Other Lipid Mediators, 70(1-2), 195-208. https://doi.org/10.1016/S0090-6980(02)00110-7

@article{a5e53b528e9d440486fafb6b9a39d82c,

title = "Effect of mifepristone on pregnancy, pregnancy-specific protein B (PSPB), progesterone, estradiol-17β, prostaglandin F2α (PGF2α) and prostaglandin E (PGE) in ovariectomized 90-day pregnant ewes",

abstract = "The objective of this experiment was to determine the effect of mifepristone, a progesterone receptor antagonist, on pregnancy and secretion of steroids, pregnancy-specific protein B (PSPB) and prostaglandins at mid-pregnancy in ewes. Ninety-day pregnant ewes were ovariectomized (OVX) and treatments were initiated 72h post-OVX. Ewes received (1) vehicle, (2) prostaglandin F2α (PGF2α, 8mg/58kg/bw, i.m.) 84h post-OVX, (3) mifepristone (50mg intrajugular at 72, 84, 96, and 108h post-OVX), (4) mifepristone (50mg)+PGF2α, (5) mifepristone (100mg intrajugular at 72, 84, 96, and 108h), and (6) mifepristone (100mg)+PGF2α. Ewes treated with vehicle or PGF2α alone did not abort (P≥0.05). But, 60, 80, 60, and 100% of ewes treated with mifepristone (50mg), mifepristone (50mg)+PGF2α, mifepristone (100mg), and mifepristone (100mg)+PGF2α, respectively, aborted (P≤0.05). Profiles of progesterone, estradiol-17β, prostaglandin E (PGE), or PSPB did not differ (P≥0.05) among treatment groups. Profiles of PGF2α of treatment groups receiving mifepristone with or without PGF2α differed (P<0.05) from vehicle or PGF2α alone-treated ewes. It is concluded that progesterone actions are necessary to suppress uterine/placental secretion of PGF2α and that maintenance of critical progesterone: estradiol-17β and PGE:PGF2α ratios are necessary for maintenance of pregnancy.",

keywords = "Ewes, Mifepristone, Pregnancy, Prostaglandin PGF",

author = "Weems, {Y. S.} and Bridges, {P. J.} and Sasser, {R. G.} and L. Ching and LeaMaster, {B. R.} and Vincent, {D. L.} and Weems, {C. W.}",

note = "Funding Information: The authors gratefully acknowledge the assistance of Mr. Alan Umaki and Mr. Armand Unabia, Waialee Livestock Research Farm for care, estrous detection, and mating of sheep used in these studies. The antiserum for the progesterone assay was kindly supplied by Dr. R.L. Butcher, West Virginia University; antiserum for PGF 2α was kindly provided by Dr. L. Levine, Brandeis University, Waltham, MA; and antiserum for PGE assays was kindly supplied by Dr. N. Mason, Lily Research Laboratories, Indianapolis, IN. Also the authors acknowledge the support of Mr. Bruce Robinson, Niihau Ranch. The authors also want to thank Mr. Eric Johnson, Hawaii Mega-Cor, Honolulu, HI for providing surgical supplies. The authors want to thank Mr. Wayne Toma for assistance with statistical analysis and computer graphics and Ms. Beverly Chang for typing of the manuscript. ",

year = "2002",

month = sep,

doi = "10.1016/S0090-6980(02)00110-7",

language = "English",

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number = "1-2",

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Weems, YS, Bridges, PJ, Sasser, RG, Ching, L, LeaMaster, BR, Vincent, DL & Weems, CW 2002, 'Effect of mifepristone on pregnancy, pregnancy-specific protein B (PSPB), progesterone, estradiol-17β, prostaglandin F_2α (PGF_2α) and prostaglandin E (PGE) in ovariectomized 90-day pregnant ewes', Prostaglandins and Other Lipid Mediators, vol. 70, no. 1-2, pp. 195-208. https://doi.org/10.1016/S0090-6980(02)00110-7

Effect of mifepristone on pregnancy, pregnancy-specific protein B (PSPB), progesterone, estradiol-17β, prostaglandin F_2α (PGF_2α) and prostaglandin E (PGE) in ovariectomized 90-day pregnant ewes. / Weems, Y. S.; Bridges, P. J.; Sasser, R. G. et al.
In: Prostaglandins and Other Lipid Mediators, Vol. 70, No. 1-2, 09.2002, p. 195-208.

Research output: Contribution to journal › Article › peer-review

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T1 - Effect of mifepristone on pregnancy, pregnancy-specific protein B (PSPB), progesterone, estradiol-17β, prostaglandin F2α (PGF2α) and prostaglandin E (PGE) in ovariectomized 90-day pregnant ewes

AU - Weems, Y. S.

AU - Bridges, P. J.

AU - Sasser, R. G.

AU - Ching, L.

AU - LeaMaster, B. R.

AU - Vincent, D. L.

AU - Weems, C. W.

N1 - Funding Information: The authors gratefully acknowledge the assistance of Mr. Alan Umaki and Mr. Armand Unabia, Waialee Livestock Research Farm for care, estrous detection, and mating of sheep used in these studies. The antiserum for the progesterone assay was kindly supplied by Dr. R.L. Butcher, West Virginia University; antiserum for PGF 2α was kindly provided by Dr. L. Levine, Brandeis University, Waltham, MA; and antiserum for PGE assays was kindly supplied by Dr. N. Mason, Lily Research Laboratories, Indianapolis, IN. Also the authors acknowledge the support of Mr. Bruce Robinson, Niihau Ranch. The authors also want to thank Mr. Eric Johnson, Hawaii Mega-Cor, Honolulu, HI for providing surgical supplies. The authors want to thank Mr. Wayne Toma for assistance with statistical analysis and computer graphics and Ms. Beverly Chang for typing of the manuscript.

PY - 2002/9

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N2 - The objective of this experiment was to determine the effect of mifepristone, a progesterone receptor antagonist, on pregnancy and secretion of steroids, pregnancy-specific protein B (PSPB) and prostaglandins at mid-pregnancy in ewes. Ninety-day pregnant ewes were ovariectomized (OVX) and treatments were initiated 72h post-OVX. Ewes received (1) vehicle, (2) prostaglandin F2α (PGF2α, 8mg/58kg/bw, i.m.) 84h post-OVX, (3) mifepristone (50mg intrajugular at 72, 84, 96, and 108h post-OVX), (4) mifepristone (50mg)+PGF2α, (5) mifepristone (100mg intrajugular at 72, 84, 96, and 108h), and (6) mifepristone (100mg)+PGF2α. Ewes treated with vehicle or PGF2α alone did not abort (P≥0.05). But, 60, 80, 60, and 100% of ewes treated with mifepristone (50mg), mifepristone (50mg)+PGF2α, mifepristone (100mg), and mifepristone (100mg)+PGF2α, respectively, aborted (P≤0.05). Profiles of progesterone, estradiol-17β, prostaglandin E (PGE), or PSPB did not differ (P≥0.05) among treatment groups. Profiles of PGF2α of treatment groups receiving mifepristone with or without PGF2α differed (P<0.05) from vehicle or PGF2α alone-treated ewes. It is concluded that progesterone actions are necessary to suppress uterine/placental secretion of PGF2α and that maintenance of critical progesterone: estradiol-17β and PGE:PGF2α ratios are necessary for maintenance of pregnancy.

AB - The objective of this experiment was to determine the effect of mifepristone, a progesterone receptor antagonist, on pregnancy and secretion of steroids, pregnancy-specific protein B (PSPB) and prostaglandins at mid-pregnancy in ewes. Ninety-day pregnant ewes were ovariectomized (OVX) and treatments were initiated 72h post-OVX. Ewes received (1) vehicle, (2) prostaglandin F2α (PGF2α, 8mg/58kg/bw, i.m.) 84h post-OVX, (3) mifepristone (50mg intrajugular at 72, 84, 96, and 108h post-OVX), (4) mifepristone (50mg)+PGF2α, (5) mifepristone (100mg intrajugular at 72, 84, 96, and 108h), and (6) mifepristone (100mg)+PGF2α. Ewes treated with vehicle or PGF2α alone did not abort (P≥0.05). But, 60, 80, 60, and 100% of ewes treated with mifepristone (50mg), mifepristone (50mg)+PGF2α, mifepristone (100mg), and mifepristone (100mg)+PGF2α, respectively, aborted (P≤0.05). Profiles of progesterone, estradiol-17β, prostaglandin E (PGE), or PSPB did not differ (P≥0.05) among treatment groups. Profiles of PGF2α of treatment groups receiving mifepristone with or without PGF2α differed (P<0.05) from vehicle or PGF2α alone-treated ewes. It is concluded that progesterone actions are necessary to suppress uterine/placental secretion of PGF2α and that maintenance of critical progesterone: estradiol-17β and PGE:PGF2α ratios are necessary for maintenance of pregnancy.

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KW - Mifepristone

KW - Pregnancy

KW - Prostaglandin PGF

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Weems YS, Bridges PJ, Sasser RG, Ching L, LeaMaster BR, Vincent DL et al. Effect of mifepristone on pregnancy, pregnancy-specific protein B (PSPB), progesterone, estradiol-17β, prostaglandin F_2α (PGF_2α) and prostaglandin E (PGE) in ovariectomized 90-day pregnant ewes. Prostaglandins and Other Lipid Mediators. 2002 Sep;70(1-2):195-208. doi: 10.1016/S0090-6980(02)00110-7