Effect of nitric oxide on single skeletal muscle fibres from the mouse

Francisco H. Andrade, Michael B. Reid, David G. Allen, Håkan Westerblad

Research output: Contribution to journalArticlepeer-review

114 Scopus citations


1. Single skeletal muscle fibres from a mouse foot muscle were used to investigate the effects of nitric oxide on contractile function. 2. We measured force production and myoplasmic free Ca2+ concentration ([Ca2+](i)) in single fibres exposed to the nitric oxide donors S-nitroso-N-acetylcysteine (SNAC) and nitroprusside. 3. The nitric oxide donors reduced myofibrillar Ca2+ sensitivity, whereas [Ca2+](i) transients were increased during submaximal tetani. Force was largely unchanged. SNAC did not, change maximum shortening velocity, the rate of force redevelopment, or force production at saturating [Ca2+](i). 4. The guanylyl cyclase inhibitor LY83583 increased tetanic [Ca2+](i) but had no effect on Ca2+ sensitivity. LY83583 did not prevent the decrease in myofibrillar Ca2+ sensitivity in response to SNAC. The oxidizer sodium nitrite increased tetanic [Ca2+](i) and decreased myofibrillar Ca2+ sensitivity. 5. We conclude that under our experimental conditions nitric oxide impairs Ca2+ activation of the actin filaments which results in decreased myofibrillar Ca2+ sensitivity.

Original languageEnglish
Pages (from-to)577-586
Number of pages10
JournalJournal of Physiology
Issue number2
StatePublished - Jun 1 1998

ASJC Scopus subject areas

  • Physiology


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