Effect of Photobiomodulation Therapy Associated With Biphasic Phosphate Calcium on Bone Repair: A Histomorphometric Study in Rats

Andréa Carvalho De Marco, Letícia Cavassini Torquato, Tatiana Camacho Ribeiro, Camilla Moretto Nunes, Daniella Vicensotto Bernardo, Clarissa Carvalho Martins Maciel, Kauê Alberto Pereira, Maria Aparecida Neves Jardini, Mauro Pedrine Santamaria

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2 Scopus citations


Introduction: This study aimed to investigate the effects of photobiomodulation (PBM) therapy associated with biphasic calcium phosphate on calvaria critical defects in rats. Methods: Forty-eight (90 days old) adult male rats (Rattus norvegicus, Albinus variation, Wistar) received critical defects of 5 mm in diameter, which were made on their skull, and they were randomly assigned into the following groups: C-blood clot, B-biphasic calcium phosphate, L-photobiomodulation therapy, and B + L-biphasic calcium phosphate + photobiomodulation therapy. A low-level a gallium aluminum arsenide (GaAlAs) laser was applied in a single dose during surgery, in a wavelength of 660 nm and total energy density of 45 J/cm2. On 30th and 60th days, the animals from each group were euthanized. Histological and histomorphometric analyses were performed. Results: In 30 days, almost all specimens (C, L, B and B + L) showed bone neoformation areas in regions near the borders of the surgical defect. In 60 days, in many specimens (C, L, B, B + L), it was possible to see a narrow neoformed bone structure along almost the whole extension of the surgical defect, though it was thinner than the original calvary bone. Data were recorded as mean ± standard deviation, and after normality was tested, a suitable statistical test was applied (α = 5%). On day 60, there was a statistically significant difference when comparing the proportion of neoformation area between group L (0.52% ± 0.13) and group B + L (0.20% ± 0.08). Group L showed a difference compared with all the groups when we compared the remaining distance between the edges of neoformed bone (C × L, P = 0.0431; B × L, P = 0.0386; L × B + L, P = 0.0352), demonstrating a great defect closure.

Original languageEnglish
Article numbere33
JournalJournal of Lasers in Medical Sciences
StatePublished - 2022

Bibliographical note

Publisher Copyright:
© 2022, Journal of Lasers in Medical Sciences.All Rights Reserved.


  • Biocompatible materials
  • Bone regeneration
  • Bone substitutes
  • Laser therapy
  • Rats

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine
  • Dentistry (miscellaneous)
  • Dermatology
  • Nephrology
  • Urology


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