Effect of protease-activated receptor 2 activation on single TRPV1 channel activities in rat vagal pulmonary sensory neurons

Qihai Gu, Lu Yuan Lee

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Protease-activated receptor 2 (PAR2) is involved in airway inflammation and airway hyperresponsiveness; both are the prominent features of asthma. Transient receptor potential vanilloid receptor 1 (TRPV1) is expressed in pulmonary sensory nerves, functions as a thermal and chemical transducer and contributes to neurogenic inflammation. Using cell-attached single-channel recordings we investigated the effect of PAR2 activation on single TRPV1 channel activities in isolated pulmonary sensory neurons. Our immunohistochemical study demonstrated the expression of PAR2 in rat vagal pulmonary sensory neurons. Our patch-clamp study further showed that intracellular application of capsaicin (0.75 μm) induced single-channel current that exhibited outward rectification in these neurons. The probability of the channel being open (Po) was significantly increased after the cells were pretreated with PAR2-activating peptide (100 μm, 2 min). Pretreatment with trypsin (0.1 μm, 2 min) also increased the single-channel Po, and the effect was completely inhibited by soybean trypsin inhibitor (0.5 μm, 3 min). In addition, the effect of PAR 2 activation was abolished by either U73122 (1 μm, 4 min), a phospholipase C inhibitor, or chelerythrine (10 μm, 4 min), a protein kinase C inhibitor. In conclusion, our data demonstrated that activation of PAR 2 upregulated single-channel activities of TRPV1 and that the effect was mediated through the protein kinase C-dependent transduction pathway.

Original languageEnglish
Pages (from-to)928-936
Number of pages9
JournalExperimental Physiology
Volume94
Issue number8
DOIs
StatePublished - Aug 2009

ASJC Scopus subject areas

  • Physiology
  • Nutrition and Dietetics
  • Physiology (medical)

Fingerprint

Dive into the research topics of 'Effect of protease-activated receptor 2 activation on single TRPV1 channel activities in rat vagal pulmonary sensory neurons'. Together they form a unique fingerprint.

Cite this