Abstract
The effect of RH-5992 (tebufenozide), a non-steroidal ecdysone agonist, on adult development of the spruce budworm, Choristoneura fumiferana, was investigated by administering the compound intrahemocoelically to pupae on days 1-6 after pupal ecdysis. At concentrations of 200 ng/pupa there was significant mortality but at doses of 50-100 ng/pupa, the emerging adults displayed wing deformities which reduced their ability to mate and oviposit. Light microscopy of the pupal wings revealed that there was degeneration of the epithelial cells, reduction in the number of veins, precocious cuticle formation and inhibition of growth of normal wing scales. Injection of RH-5992 into pupae resulted in a dose dependent induction of mRNA for ecdysone-induced transcription factor, Choristoneura hormone receptor 3 (CHR3). These results suggest that the pupae respond to RH-5992 in a manner similar to larvae. However, the effects are not expressed overtly and are camouflaged by the pharmacological effects.
| Original language | English |
|---|---|
| Pages (from-to) | 225-231 |
| Number of pages | 7 |
| Journal | Insect Biochemistry and Molecular Biology |
| Volume | 32 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2002 |
Bibliographical note
Funding Information:The authors gratefully acknowledge and thank Rohm and Haas for providing samples of RH-5992, Drs T.S. Dhadialla and S.S. Sohi for their critical reading, and Ms K. Jamieson for editorial assistance. This research was supported by a Canadian Forest Service/Natural Sciences and Engineering Research Council Research Partnership Program Award CRD No. 0192158, with Rohm and Haas and the University of Guelph. Dr G. Smagghe acknowledges a post-doctoral fellowship from the Fund of Scientific Research (FWO, Belgium).
Keywords
- CHR3
- Egg laying
- Fecundity
- RH-5992
- Tebufenozide
- Wing deformities
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Insect Science