Effect of silver nanoparticle surface coating on bioaccumulation and reproductive toxicity in earthworms (Eisenia fetida)

William A. Shoults-Wilson; Brian C. Reinsch; Olga V. Tsyusko; Paul M. Bertsch; Gregory V. Lowry; Jason M. Unrine

doi:10.3109/17435390.2010.537382

Effect of silver nanoparticle surface coating on bioaccumulation and reproductive toxicity in earthworms (Eisenia fetida)

William A. Shoults-Wilson, Brian C. Reinsch, Olga V. Tsyusko, Paul M. Bertsch, Gregory V. Lowry, Jason M. Unrine

Research output: Contribution to journal › Article › peer-review

177 Scopus citations

Abstract

The purpose of this study was to investigate the effect of surface coating on the toxicity of silver nanoparticles (Ag NPs) soil. Earthworms (Eisenia fetida) were exposed to AgNO ₃ and Ag NPs with similar size ranges coated with either polyvinylpyrrolidone (hydrophilic) or oleic acid (amphiphilic) during a standard sub-chronic reproduction toxicity test. No significant effects on growth or mortality were observed within any of the test treatments. Significant decreases in reproduction were seen in earthworms exposed to AgNO ₃, (94.21 mg kg ^-1) as well as earthworms exposed to Ag NPs with either coating (727.6 mg kg ^-1 for oleic acid and 773.3 mg kg ^-1 for polyvinylpyrrolidone). The concentrations of Ag NPs at which effects were observed are much higher than predicted concentrations of Ag NPs in sewage sludge amended soils; however, the concentrations at which adverse effects of AgNO ₃ were observed are similar to the highest concentrations of Ag presently observed in sewage sludge in the United States. Earthworms accumulated Ag in a concentration-dependent manner from all Ag sources, with more Ag accumulating in tissues from AgNO ₃ compared to earthorms exposed to equivalent concentrations of Ag NPs. No differences were observed in Ag accumulation or toxicity between earthworms exposed to Ag NPs with polyvinylpyrrolidone or oleic acid coatings.

Original language	English
Pages (from-to)	432-444
Number of pages	13
Journal	Nanotoxicology
Volume	5
Issue number	3
DOIs	https://doi.org/10.3109/17435390.2010.537382
State	Published - Sep 2011

Bibliographical note

Funding Information:
Declaration of interest: Major funding for this research was provided by the United States Environmental Protection Agency (U.S. EPA) through Science to Achieve Results Grant # RD 833335 and through support of the National Science Foundation (NSF) and U.S. EPA for the Center for Environmental Implications of Nanotechnology (CEINT). It was also funded in part by the National Science Foundation (BES-0608646 and EF-0830093), and the U.S. EPA R833326. The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper.

Keywords

Bioaccumulation
Eisenia fetida
Reproductive toxicity
Silver nanoparticles
X-ray absorption spectroscopy

ASJC Scopus subject areas

Biomedical Engineering
Toxicology

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10.3109/17435390.2010.537382

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title = "Effect of silver nanoparticle surface coating on bioaccumulation and reproductive toxicity in earthworms (Eisenia fetida)",

abstract = "The purpose of this study was to investigate the effect of surface coating on the toxicity of silver nanoparticles (Ag NPs) soil. Earthworms (Eisenia fetida) were exposed to AgNO 3 and Ag NPs with similar size ranges coated with either polyvinylpyrrolidone (hydrophilic) or oleic acid (amphiphilic) during a standard sub-chronic reproduction toxicity test. No significant effects on growth or mortality were observed within any of the test treatments. Significant decreases in reproduction were seen in earthworms exposed to AgNO 3, (94.21 mg kg -1) as well as earthworms exposed to Ag NPs with either coating (727.6 mg kg -1 for oleic acid and 773.3 mg kg -1 for polyvinylpyrrolidone). The concentrations of Ag NPs at which effects were observed are much higher than predicted concentrations of Ag NPs in sewage sludge amended soils; however, the concentrations at which adverse effects of AgNO 3 were observed are similar to the highest concentrations of Ag presently observed in sewage sludge in the United States. Earthworms accumulated Ag in a concentration-dependent manner from all Ag sources, with more Ag accumulating in tissues from AgNO 3 compared to earthorms exposed to equivalent concentrations of Ag NPs. No differences were observed in Ag accumulation or toxicity between earthworms exposed to Ag NPs with polyvinylpyrrolidone or oleic acid coatings.",

keywords = "Bioaccumulation, Eisenia fetida, Reproductive toxicity, Silver nanoparticles, X-ray absorption spectroscopy",

author = "Shoults-Wilson, {William A.} and Reinsch, {Brian C.} and Tsyusko, {Olga V.} and Bertsch, {Paul M.} and Lowry, {Gregory V.} and Unrine, {Jason M.}",

note = "Funding Information: Declaration of interest: Major funding for this research was provided by the United States Environmental Protection Agency (U.S. EPA) through Science to Achieve Results Grant # RD 833335 and through support of the National Science Foundation (NSF) and U.S. EPA for the Center for Environmental Implications of Nanotechnology (CEINT). It was also funded in part by the National Science Foundation (BES-0608646 and EF-0830093), and the U.S. EPA R833326. The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper.",

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AU - Bertsch, Paul M.

AU - Lowry, Gregory V.

AU - Unrine, Jason M.

N1 - Funding Information: Declaration of interest: Major funding for this research was provided by the United States Environmental Protection Agency (U.S. EPA) through Science to Achieve Results Grant # RD 833335 and through support of the National Science Foundation (NSF) and U.S. EPA for the Center for Environmental Implications of Nanotechnology (CEINT). It was also funded in part by the National Science Foundation (BES-0608646 and EF-0830093), and the U.S. EPA R833326. The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper.

PY - 2011/9

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N2 - The purpose of this study was to investigate the effect of surface coating on the toxicity of silver nanoparticles (Ag NPs) soil. Earthworms (Eisenia fetida) were exposed to AgNO 3 and Ag NPs with similar size ranges coated with either polyvinylpyrrolidone (hydrophilic) or oleic acid (amphiphilic) during a standard sub-chronic reproduction toxicity test. No significant effects on growth or mortality were observed within any of the test treatments. Significant decreases in reproduction were seen in earthworms exposed to AgNO 3, (94.21 mg kg -1) as well as earthworms exposed to Ag NPs with either coating (727.6 mg kg -1 for oleic acid and 773.3 mg kg -1 for polyvinylpyrrolidone). The concentrations of Ag NPs at which effects were observed are much higher than predicted concentrations of Ag NPs in sewage sludge amended soils; however, the concentrations at which adverse effects of AgNO 3 were observed are similar to the highest concentrations of Ag presently observed in sewage sludge in the United States. Earthworms accumulated Ag in a concentration-dependent manner from all Ag sources, with more Ag accumulating in tissues from AgNO 3 compared to earthorms exposed to equivalent concentrations of Ag NPs. No differences were observed in Ag accumulation or toxicity between earthworms exposed to Ag NPs with polyvinylpyrrolidone or oleic acid coatings.

AB - The purpose of this study was to investigate the effect of surface coating on the toxicity of silver nanoparticles (Ag NPs) soil. Earthworms (Eisenia fetida) were exposed to AgNO 3 and Ag NPs with similar size ranges coated with either polyvinylpyrrolidone (hydrophilic) or oleic acid (amphiphilic) during a standard sub-chronic reproduction toxicity test. No significant effects on growth or mortality were observed within any of the test treatments. Significant decreases in reproduction were seen in earthworms exposed to AgNO 3, (94.21 mg kg -1) as well as earthworms exposed to Ag NPs with either coating (727.6 mg kg -1 for oleic acid and 773.3 mg kg -1 for polyvinylpyrrolidone). The concentrations of Ag NPs at which effects were observed are much higher than predicted concentrations of Ag NPs in sewage sludge amended soils; however, the concentrations at which adverse effects of AgNO 3 were observed are similar to the highest concentrations of Ag presently observed in sewage sludge in the United States. Earthworms accumulated Ag in a concentration-dependent manner from all Ag sources, with more Ag accumulating in tissues from AgNO 3 compared to earthorms exposed to equivalent concentrations of Ag NPs. No differences were observed in Ag accumulation or toxicity between earthworms exposed to Ag NPs with polyvinylpyrrolidone or oleic acid coatings.

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KW - Reproductive toxicity

KW - Silver nanoparticles

KW - X-ray absorption spectroscopy

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Effect of silver nanoparticle surface coating on bioaccumulation and reproductive toxicity in earthworms (Eisenia fetida)

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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