Effect of stover fraction and storage method on glucose production during enzymatic hydrolysis

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


One avenue for overcoming the economic challenges associated with the production of ethanol from renewable resources is to reduce the cost of the biomass feedstock. The balance between storage costs and benefits depend on the storage method and composition changes of individual stover fractions. Corn stover from bales stored inside and outside of a barn was separated into an interior and exterior layer after approximately 10 months of storage. The cobs, stalks, and leaves and husks were separated, dried, and ground through a 2 mm screen. Stover, sodium acetate (buffer), cellulase, and deionized water were added to 125 ml flasks. The mixture was held at 50°C in an incubator and samples taken for glucose determination. The average glucose concentration after 60 h of hydrolysis from cobs, leaves and husks, and stalks was 10.5, 9.6, and 3.1 g/l, respectively. Cobs, leaves, and husks produced over 300% more glucose than stalks. Storage outside of the barn decreased the glucose production from individual stover components between 4% and 8%. The effect of stover fraction type on glucose production was significant, while the storage treatment effect was not significant. Fractionation of corn stover may be a method to increase the value of corn stover as a feedstock for glucose production.

Original languageEnglish
Pages (from-to)269-274
Number of pages6
JournalBioresource Technology
Issue number3
StatePublished - May 2004

Bibliographical note

Funding Information:
The authors wish to acknowledge the financial support from Oak Ridge National Laboratory under contract number 4000011647. The authors thank Alltech Inc. for providing cellulase enzymes. The authors would also like to thank Wei Chen and Wayne Ingram for performing laboratory work. This article is published with the approval of the Director of the Kentucky Agricultural Experiment Station and designated paper number 03-05-112.


  • Bale
  • Biomass
  • Fractionation
  • Glucose
  • Storage

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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