Effect of temperature on heart rate for phaenicia sericata and drosophila melanogaster with altered expression of the trpa1 receptors

Nicole T. Marguerite, Jate Bernard, Douglas A. Harrison, David Harris, Robin L. Cooper

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The transient receptor potential (TrpA—ankyrin) receptor has been linked to pathological conditions in cardiac function in mammals. To better understand the function of the TrpA1 in regulation of the heart, a Drosophila melanogaster model was used to express TrpA1 in heart and body wall muscles. Heartbeat of in intact larvae as well as hearts in situ, devoid of hormonal and neural input, indicate that strong over-expression of TrpA1 in larvae at 30 or 37C stopped the heart from beating, but in a diastolic state. Cardiac function recovered upon cooling after short exposure to high temperature. Parental control larvae (UAS-TrpA1) increased heart rate transiently at 30 and 37C but slowed at 37C within 3 min for in-situ preparations, while in-vivo larvae maintained a constant heart rate. The in-situ preparations maintained an elevated rate at 30C. The heartbeat in the TrpA1-expressing strains could not be revived at 37C with serotonin. Thus, TrpA1 activation may have allowed enough Ca2+ influx to activate K(Ca) channels into a form of diastolic stasis. TrpA1 activation in body wall muscle confirmed a depolarization of membrane. In contrast, blowfly Phaenicia sericata larvae increased heartbeat at 30 and 37C, demonstrating greater cardiac thermotolerance.

Original languageEnglish
Article number38
Pages (from-to)1-17
Number of pages17
JournalInsects
Volume12
Issue number1
DOIs
StatePublished - Jan 2021

Bibliographical note

Funding Information:
Chellgren Endowed Professor fund from the University of Kentucky to R.L.C. for supplies.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Drosophila
  • Heart
  • TRPA
  • Temperature

ASJC Scopus subject areas

  • Insect Science

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