Effect of the aromatase inhibitor CGS-16949A on pregnancy and secretion of progesterone, estradiol-17β, prostaglandins E and F (PGE; PGF) and pregnancy specific protein B (PSPB) in 90-day ovariectomized pregnant ewes

Y. S. Weems, P. J. Bridges, B. R. LeaMaster, R. G. Sasser, L. Ching, C. W. Weems

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15 Scopus citations

Abstract

The aromatase inhibitor CGS-16949A was used to determine whether CGS-16949A altered secretion of progesterone, estradiol-17β, PGE (PGE1 + PGE2), PGF and PSPB. Ninety day pregnant ewes were ovariectomized and received vehicle, PGF, CGS-16949A or PGF+CGS-16949A. None of the ewes treated with PGF, CGS-16949A or PGF+CGS-16949A aborted (P ≥ 0.05) during the 108-h experimental period. Treatment with CGS-16949A lowered (P ≤ 0.05) progesterone in jugular venous plasma but concentrations of progesterone were not affected (P ≥ 0.05) by treatment with PGF. Concentrations of estradiol-17β and PSPB in jugular venous plasma and PGE in inferior vena cava plasma were decreased (P ≤ 0.05) by treatment with CGS-16949A. Concentrations of PGF in inferior vena cava plasma were not affected (P ≥ 0.05) by treatment with CGS-16949A. Decreases in estradiol-17β occurred before decreases in PSPB, which was then followed by decreases in PGE (P ≤ 0.05). It is concluded that these data support the hypothesis that estradiol-17β regulates placental secretion of PSPB; PSPB regulates placental secretion of PGE; and PGE regulates placental secretion of progesterone during mid-pregnancy in ewes.

Original languageEnglish
Pages (from-to)77-88
Number of pages12
JournalProstaglandins and Other Lipid Mediators
Volume66
Issue number2
DOIs
StatePublished - 2001

Bibliographical note

Funding Information:
The authors thank Ms. Beverly Chang and Mr. Wayne Toma for typing of the manuscript and computer graphics. This paper is Journal Series No. 4525 of the Hawaii Institute of Tropical Agriculture and Human Resources. This research was supported in part by the United States Department of Agriculture under CSREES Special Grant No. 95-34135-1776 to C.W. Weems managed by the Pacific Basin Advisory Group, USDA Hatch Project 259 (USDA W-112) and Hatch Project R-876 (R.G.S.). The authors express their gratitude to Dr. Norm Mason (Eli Lilly), Dr. R.L. Butcher (West Virginia University) and Dr. Lawrence Levine (Brandeis University) for antisera to PGE 2 , progesterone and estradiol-17β and PGF 2α , respectively. The authors want to thank Mr. Eric Johnson, Hawaii Mega-Cor Inc. and Mr. Bruce Robinson, Niihau Ranch for their support. The authors thank Dr. Ajay S. Bhatnagar and Ciba Geigy Ltd. Basel Switzerland for the generous donation of CGS-16949A.

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Pharmacology
  • Cell Biology

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