@article{c6700eb7895b4416ac7f6e6da4f2b957,
title = "Effect of thromboxane synthase inhibition on eicosanoid levels and blood flow in ischemic rat brain",
abstract = "Reperfusion of ischemic brain is associated with production of thromboxane A2 (TXA2), a proaggregatory vasoconstrictor. We used an animal model of transient forebrain ischemia to study the effects of 1-benzylimidazole (1-BI), a selective inhibitor of thromboxane synthase, upon cerebral elcosanoid levels and cerebral blood flow. Male Wistar rats were subjected to 30 minutes of four-vessel occlusion. The mean±SEM brain level of TXB2, the stable metabolite of TXA2, determined after 60 minutes of reperfusion was 101±20 pg/mg brain protein in five ischemic control rats. Infusion of 10 μg/g 1-BI reduced mean±SEM cerebral TXB2 concentration to 11±3 pg/mg brain protein in five rats (p≤0.002). Mean±SEM hemispheric cerebral blood flow measured in four ischemic control rats after 60 minutes of reperfusion was 42±9 ml/100 g brain/min compared with 104±13 ml/100 g brain/min in three 1-BI-treated rats (p≤0.001). Mean±SEM hippocampal blood flow in four ischemic control rats after 60 minutes of reperfusion was 51±14 ml/100 g brain/min compared with 125±25 ml/100 g brain/min in three 1-BI-treated rats (p≤0.04). We conclude that selective inhibition of thromboxane synthase may alleviate ischemic brain damage by reducing cerebral TXA2 concentrations and elevating cerebral blood flow.",
keywords = "Cerebral blood flow, Cerebral ischemia, Rats, Thromboxane synthase",
author = "Pettigrew, {L. C.} and Grotta, {J. C.} and Rhoades, {H. M.} and Wu, {K. K.}",
year = "1989",
month = may,
doi = "10.1161/01.STR.20.5.627",
language = "English",
volume = "20",
pages = "627--632",
number = "5",
}