Effect of tunicamycin on peroxidase release by cultured peanut suspension cells

K. Ravi, C. Hu, P. S. Reddi, R. B. Van Huystee

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33 Scopus citations


Ravi, K Hu, C., Reddi, P. S. and van H uystee, R. B. 1986. Effect of tunicamycin on peroxidase release by cultured peanut suspension cells.-J. exp. Bol 37: 1708-1715.Tunicamycin added at 50 μg cm-3 of medium to a suspension culture of peanut cells inhibited pcroxid.ase release. This could be measured by a loss of peroxidase accumulation not only in terms of enzyme activity but also through a reduction in labelled immunoprecipitated product from the medium following incubation of the cells with 35S-methionine or 3H-mannosc. In addition, the antibodies raised against a purilled cationic peanut peroxidase were employed in isolation of radioactive fractions that were analysed subsequently by electrophoresis followed by fluorography. This confirmed that no newly-synthesized peroxidase was released and that the newly-synthesized peroxidase in tunicainycin-treated cells possessed a slightly lower molecular weight than that in control cells. This may be due to the inhibition, by tunicamycin, of glycosylation of N-linked glycoproteins as demonstrated by the lower labelling with 3H-mannose of lipid-linked oligosacchandes.

Original languageEnglish
Pages (from-to)1708-1715
Number of pages8
JournalJournal of Experimental Botany
Issue number11
StatePublished - Nov 1986

Bibliographical note

Funding Information:
ACKNOWLEDGEMENTS Research funds for this study were obtained from NSERCC and a contract of D.S.S. Government of Canada to R. B. van Huystee. The help of Mr A. Tam, Dept. of Plant Sciences is gratefully acknowledged as is the use of facilities in the Dept. of Biochem., U.W.O. under the direction of Dr K. K. Carroll for some of the lipid related analysis.


  • 3H-mannose
  • Giycoprotein
  • Peroxidase
  • Radio-immunology
  • Tunicamycin

ASJC Scopus subject areas

  • Physiology
  • Plant Science


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