Phenobarbital (PB) is an efficacious and well-studied hepatic tumor promoting agent. Nuclear factor-κB (NF-κB) is a transcription factor activated by reactive oxygen and is involved in cell proliferation and apoptosis. We previously found that PB activates NF-κB and that dietary vitamin E is effective in decreasing PB-induced NF-κB DNA binding. We therefore hypothesized that dietary vitamin E influences PB-induced changes in cell proliferation and apoptosis through its action on NF-κB. NF-κB1 deficient mice (p50-/-) and wild-type B6129 mice were fed a purified diet containing 10 or 250. ppm vitamin E (α-tocopherol acetate) for 28. days. At that time, half of the wild-type and half of the p50-/- mice were placed on the same diet with 0.05% PB for 10. days. Compared to wild-type mice, the p50-/- mice had higher levels of cell proliferation and apoptosis. Cell proliferation was significantly increased by PB, but vitamin E did not affect hepatic cell proliferation. Apoptosis was not changed in mice fed PB, and there was no significant difference in apoptosis between control and high vitamin E treated mice. Thus, vitamin E does not appear to influence cell growth parameters in either wild-type or p50-/- mice.
|Number of pages||4|
|Journal||Food and Chemical Toxicology|
|State||Published - Oct 2011|
Bibliographical noteFunding Information:
This work was supported by the National Institute of Environmental Health Sciences ( ES11480 ), the China Scholarship Council, and the Kentucky Agricultural Experiment Station. None of these funding sources played a role in study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the paper for publication.
- Cell proliferation
- Vitamin E
ASJC Scopus subject areas
- Food Science