Effectiveness of siRNA uptake in target tissues by various delivery methods

Shawn D. Larson, Lindsey N. Jackson, L. Andy Chen, Piotr G. Rychahou, B. Mark Evers

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Background: RNA interference offers clinical potential as a therapeutic modality for a variety of diseases; the efficacy of in vivo delivery remains poorly understood. The purpose of our study was to compare and contrast short interfering RNA (siRNA) uptake in vivo using various delivery techniques. Methods: DY547- and rhodamine-labeled siRNA was administered to mice by one of four delivery methods: (1) hydrodynamic intravenous tail vein injection, (2) standard intravenous tail vein injection, (3) intraperitoneal injection, and (4) rectal administration. Mice were killed over a time course; representative tissue samples were collected and analyzed using fluorescent microscopy to determine siRNA uptake. Results: siRNA uptake was noted in the liver, kidney, pancreas, spleen, and bone marrow by both hydrodynamic and standard IV injection. siRNA uptake was detected in the spleen, liver, and bone marrow after intraperitoneal administration. Rectal administration resulted in siRNA uptake in the spleen, bone marrow, and colon. Conclusion: Our results demonstrate differential siRNA uptake depending on delivery technique. Importantly, our results demonstrate the potential of siRNA as a systemic therapeutic option in vivo for selected disease processes.

Original languageEnglish
Pages (from-to)262-269
Number of pages8
JournalSurgery
Volume142
Issue number2
DOIs
StatePublished - Aug 2007

Bibliographical note

Funding Information:
Supported by grants RO1CA104748, RO1DK48498, PO1DK35608, and T32DK07639 from the National Institutes of Health and a Jeane B. Kempner Scholar award (to S.D.L.).

ASJC Scopus subject areas

  • Surgery

Fingerprint

Dive into the research topics of 'Effectiveness of siRNA uptake in target tissues by various delivery methods'. Together they form a unique fingerprint.

Cite this