Effects of altering aminoglycoside structures on bacterial resistance enzyme activities

Keith D. Green; Wenjing Chen; Sylvie Garneau-Tsodikova

doi:10.1128/AAC.00312-11

Effects of altering aminoglycoside structures on bacterial resistance enzyme activities

Keith D. Green, Wenjing Chen, Sylvie Garneau-Tsodikova

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Aminoglycoside-modifying enzymes (AMEs) constitute the most prevalent mechanism of resistance to aminoglycosides by bacteria. We show that aminoglycosides can be doubly modified by the sequential actions of AMEs, with the activity of the second AME in most cases unaffected, decreased, or completely abolished. We demonstrate that the bifunctional enzyme AAC(3)-Ib/AAC(6′)-Ib′ can diacetylate gentamicin. Since single acetylation does not always inactivate the parent drugs completely, two modifications likely provide morerobust inactivation in vivo.

Original language	English
Pages (from-to)	3207-3213
Number of pages	7
Journal	Antimicrobial Agents and Chemotherapy
Volume	55
Issue number	7
DOIs	https://doi.org/10.1128/AAC.00312-11
State	Published - Jul 2011

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/AAC.00312-11

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AB - Aminoglycoside-modifying enzymes (AMEs) constitute the most prevalent mechanism of resistance to aminoglycosides by bacteria. We show that aminoglycosides can be doubly modified by the sequential actions of AMEs, with the activity of the second AME in most cases unaffected, decreased, or completely abolished. We demonstrate that the bifunctional enzyme AAC(3)-Ib/AAC(6′)-Ib′ can diacetylate gentamicin. Since single acetylation does not always inactivate the parent drugs completely, two modifications likely provide morerobust inactivation in vivo.

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Effects of altering aminoglycoside structures on bacterial resistance enzyme activities

Abstract

ASJC Scopus subject areas

UN SDGs

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