Effects of chromium(III) picolinate on cortisol and DHEAs secretion in H295R human adrenocortical cells

Beob G. Kim, Julye M. Adams, Brian A. Jackson, Merlin D. Lindemann

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Dietary chromium(III) picolinate (CrPic) effects on circulating steroid hormones have been reported in various experimental animals. However, direct effects of CrPic on adrenocortical steroidogenesis are uncertain. Therefore, the objective was to determine the effects of CrPic on Cortisol and dehydroepiandrosterone sulfate (DHEAs) secretion from H295R cells. In experiment 1, a 24-h exposure to CrPic (0 to 200 μM) had both linear (p< 0.001) and quadratic (p<0.001) effects on cortisol secretion from forskolin-stimulated cells with the highest Cortisol secretion at 0.1 μM of CrPic and the lowest at 200 μM of CrPic. In experiment 2, a 48-h exposure to CrPic (200 μM) decreased cortisol (p<0.07) release from forskolin-stimulated cells during a 24-h collection period. In experiment 3, a 48-h exposure to CrPic (100 μM) decreased Cortisol p<0.05) and DHEAs (p<0.01) from forskolinstimulated cells during a 24-h sampling period. In experiment 4, a 24-h exposure to forskolin followed by a 24-h exposure to both forskolin and CrPic (100 and 200 μM) decreased both Cortisol and DHEAs secretion (p<0.01). This study suggests that at high concentrations, CrPic inhibits aspects of steroidogenesis in agonist-stimulated adrenocortical cells.

Original languageEnglish
Pages (from-to)171-180
Number of pages10
JournalBiological Trace Element Research
Issue number2
StatePublished - Feb 2010

Bibliographical note

Funding Information:
Acknowledgements The authors thank Mrs. S. Hayes, University of Kentucky, for assistance in radioimmunoassay, and Dr. R. A. Anderson, USDA-ARS, and Dr. J. C. Matthews, University of Kentucky, for helpful comments. Special appreciation is expressed to Nutrition 21, Purchase, NY, for partial support for this study. During this research, B. G. Kim was a recipient of the Presidential Graduate Fellowship and the Kentucky Opportunity Fellowship at the University of Kentucky.


  • Adrenocorticoid
  • Chromium
  • Cortisol
  • Dheas
  • H295R
  • Steroid

ASJC Scopus subject areas

  • Biochemistry, medical
  • Biochemistry
  • Clinical Biochemistry
  • Inorganic Chemistry
  • Endocrinology, Diabetes and Metabolism


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