Effects of dietary supplementation with fish oil on in vivo production of inflammatory mediators in clinically normal dogs

Casey J. LeBlanc, David W. Horohov, John E. Bauer, Giselle Hosgood, Glenna E. Mauldin

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Objective - To evaluate the effect of diets enriched with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on in vivo production of interleukin (IL)-1, IL-6, tumor necrosis factor (TNF)-α, prostaglandin E2 (PGE2), and platelet-activating factor (PAF) in dogs. Animals - 15 young healthy dogs. Procedures - Dogs were randomly allocated to receive an isocaloric ration supplemented with sunflower oil (n = 5), fish oil (5), or fish oil plus vitamin E (5) for 12 weeks. At week 12, in vivo production of inflammatory mediators was evaluated in serum at multiple time points for 6 hours following stimulation with IV administration of lipopolysaccharide (LPS). Results - Serum activity or concentration (area under the curve) of IL-1, IL-6, and PGE2 significantly increased after LPS injection in all groups but to a lesser extent in dogs receiving the fish oil diet, compared with results for dogs receiving the sunflower oil diet. Serum activity of TNF-α and PAF concentration also increased significantly after LPS injection in all groups but did not differ significantly among groups. Conclusions and Clinical Rellevance - A fish oil-enriched diet consisting of 1.75 g of EPA/kg of diet and 2.2 g of DHA/kg of diet (dry-matter basis) with an n-6:n-3 fatty acid ratio of 3.4:1 was associated with significant reductions in serum PGE concentrations and 10 and IL-6 activities. Results supported the use of EPA- and DHA-enriched diets as part of antiinflammatory treatments for dogs with chronic inflammatory diseases. Additional studies in affected dogs are warranted to further evaluate beneficial anti-inflammatory effects of EPA- and DHA-enriched diets.

Original languageEnglish
Pages (from-to)486-493
Number of pages8
JournalAmerican Journal of Veterinary Research
Volume69
Issue number4
DOIs
StatePublished - Apr 2008

ASJC Scopus subject areas

  • Veterinary (all)

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