Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologies

Venkata Ramesh Pallapolu; Y. Y. Lee; Rebecca J. Garlock; Venkatesh Balan; Bruce E. Dale; Youngmi Kim; Nathan S. Mosier; Michael R. Ladisch; Matthew Falls; Mark T. Holtzapple; Rocio Sierra-Ramirez; Jian Shi; Mirvat A. Ebrik; Tim Redmond; Bin Yang; Charles E. Wyman; Bryon S. Donohoe; Todd B. Vinzant; Richard T. Elander; Bonnie Hames; Steve Thomas; Ryan E. Warner

doi:10.1016/j.biortech.2011.03.085

Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologies

Venkata Ramesh Pallapolu, Y. Y. Lee, Rebecca J. Garlock, Venkatesh Balan, Bruce E. Dale, Youngmi Kim, Nathan S. Mosier, Michael R. Ladisch, Matthew Falls, Mark T. Holtzapple, Rocio Sierra-Ramirez, Jian Shi, Mirvat A. Ebrik, Tim Redmond, Bin Yang, Charles E. Wyman, Bryon S. Donohoe, Todd B. Vinzant, Richard T. Elander, Bonnie HamesSteve Thomas, Ryan E. Warner

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

The objective of this work is to investigate the effects of cellulase loading and β-glucosidase supplementation on enzymatic hydrolysis of pretreated Dacotah switchgrass. To assess the difference among various pretreatment methods, the profiles of sugars and intermediates were determined for differently treated substrates. For all pretreatments, 72h glucan/xylan digestibilities increased sharply with enzyme loading up to 25mg protein/g-glucan, after which the response varied depending on the pretreatment method. For a fixed level of enzyme loading, dilute sulfuric acid (DA), SO ₂, and Lime pretreatments exhibited higher digestibility than the soaking in aqueous ammonia (SAA) and ammonia fiber expansion (AFEX). Supplementation of Novozyme-188 to Spezyme-CP improved the 72h glucan digestibility only for the SAA treated samples. The effect of β-glucosidase supplementation was discernible only at the early phase of hydrolysis where accumulation of cellobiose and oligomers is significant. Addition of β-glucosidase increased the xylan digestibility of alkaline treated samples due to the β-xylosidase activity present in Novozyme-188.

Original language	English
Pages (from-to)	11115-11120
Number of pages	6
Journal	Bioresource Technology
Volume	102
Issue number	24
DOIs	https://doi.org/10.1016/j.biortech.2011.03.085
State	Published - Dec 2011

Bibliographical note

Funding Information:
We gratefully acknowledge the financial support provided by the Office of the Biomass Program of DOE (Contract: DE-FG36-07GO17102 ). We also wish to thank the members of CAFI team for their useful suggestions and collaboration, Genencor-Dansico (Paulo Alto, CA) for providing the enzymes used in this research and Ceres, Inc. (Thousand Oaks, CA) for providing switchgrass feedstocks.

Funding

We gratefully acknowledge the financial support provided by the Office of the Biomass Program of DOE (Contract: DE-FG36-07GO17102 ). We also wish to thank the members of CAFI team for their useful suggestions and collaboration, Genencor-Dansico (Paulo Alto, CA) for providing the enzymes used in this research and Ceres, Inc. (Thousand Oaks, CA) for providing switchgrass feedstocks.

Funders	Funder number
U.S. Department of Energy Oak Ridge National Laboratory U.S. Department of Energy National Science Foundation National Energy Research Scientific Computing Center	DE-FG36-07GO17102
U.S. Department of Energy Oak Ridge National Laboratory U.S. Department of Energy National Science Foundation National Energy Research Scientific Computing Center

Keywords

Cellulase
Digestibility
Pretreatment
Switchgrass
β-Glucosidase

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.biortech.2011.03.085

Cite this

Pallapolu, V. R., Lee, Y. Y., Garlock, R. J., Balan, V., Dale, B. E., Kim, Y., Mosier, N. S., Ladisch, M. R., Falls, M., Holtzapple, M. T., Sierra-Ramirez, R., Shi, J., Ebrik, M. A., Redmond, T., Yang, B., Wyman, C. E., Donohoe, B. S., Vinzant, T. B., Elander, R. T., ... Warner, R. E. (2011). Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologies. Bioresource Technology, 102(24), 11115-11120. https://doi.org/10.1016/j.biortech.2011.03.085

@article{5cce4a4e10b84c81b018ba09577a457f,

title = "Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologies",

abstract = "The objective of this work is to investigate the effects of cellulase loading and β-glucosidase supplementation on enzymatic hydrolysis of pretreated Dacotah switchgrass. To assess the difference among various pretreatment methods, the profiles of sugars and intermediates were determined for differently treated substrates. For all pretreatments, 72h glucan/xylan digestibilities increased sharply with enzyme loading up to 25mg protein/g-glucan, after which the response varied depending on the pretreatment method. For a fixed level of enzyme loading, dilute sulfuric acid (DA), SO 2, and Lime pretreatments exhibited higher digestibility than the soaking in aqueous ammonia (SAA) and ammonia fiber expansion (AFEX). Supplementation of Novozyme-188 to Spezyme-CP improved the 72h glucan digestibility only for the SAA treated samples. The effect of β-glucosidase supplementation was discernible only at the early phase of hydrolysis where accumulation of cellobiose and oligomers is significant. Addition of β-glucosidase increased the xylan digestibility of alkaline treated samples due to the β-xylosidase activity present in Novozyme-188.",

keywords = "Cellulase, Digestibility, Pretreatment, Switchgrass, β-Glucosidase",

author = "Pallapolu, \{Venkata Ramesh\} and Lee, \{Y. Y.\} and Garlock, \{Rebecca J.\} and Venkatesh Balan and Dale, \{Bruce E.\} and Youngmi Kim and Mosier, \{Nathan S.\} and Ladisch, \{Michael R.\} and Matthew Falls and Holtzapple, \{Mark T.\} and Rocio Sierra-Ramirez and Jian Shi and Ebrik, \{Mirvat A.\} and Tim Redmond and Bin Yang and Wyman, \{Charles E.\} and Donohoe, \{Bryon S.\} and Vinzant, \{Todd B.\} and Elander, \{Richard T.\} and Bonnie Hames and Steve Thomas and Warner, \{Ryan E.\}",

note = "Funding Information: We gratefully acknowledge the financial support provided by the Office of the Biomass Program of DOE (Contract: DE-FG36-07GO17102 ). We also wish to thank the members of CAFI team for their useful suggestions and collaboration, Genencor-Dansico (Paulo Alto, CA) for providing the enzymes used in this research and Ceres, Inc. (Thousand Oaks, CA) for providing switchgrass feedstocks.",

year = "2011",

month = dec,

doi = "10.1016/j.biortech.2011.03.085",

language = "English",

volume = "102",

pages = "11115--11120",

number = "24",

}

Pallapolu, VR, Lee, YY, Garlock, RJ, Balan, V, Dale, BE, Kim, Y, Mosier, NS, Ladisch, MR, Falls, M, Holtzapple, MT, Sierra-Ramirez, R, Shi, J, Ebrik, MA, Redmond, T, Yang, B, Wyman, CE, Donohoe, BS, Vinzant, TB, Elander, RT, Hames, B, Thomas, S & Warner, RE 2011, 'Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologies', Bioresource Technology, vol. 102, no. 24, pp. 11115-11120. https://doi.org/10.1016/j.biortech.2011.03.085

TY - JOUR

T1 - Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologies

AU - Pallapolu, Venkata Ramesh

AU - Lee, Y. Y.

AU - Garlock, Rebecca J.

AU - Balan, Venkatesh

AU - Dale, Bruce E.

AU - Kim, Youngmi

AU - Mosier, Nathan S.

AU - Ladisch, Michael R.

AU - Falls, Matthew

AU - Holtzapple, Mark T.

AU - Sierra-Ramirez, Rocio

AU - Shi, Jian

AU - Ebrik, Mirvat A.

AU - Redmond, Tim

AU - Yang, Bin

AU - Wyman, Charles E.

AU - Donohoe, Bryon S.

AU - Vinzant, Todd B.

AU - Elander, Richard T.

AU - Hames, Bonnie

AU - Thomas, Steve

AU - Warner, Ryan E.

N1 - Funding Information: We gratefully acknowledge the financial support provided by the Office of the Biomass Program of DOE (Contract: DE-FG36-07GO17102 ). We also wish to thank the members of CAFI team for their useful suggestions and collaboration, Genencor-Dansico (Paulo Alto, CA) for providing the enzymes used in this research and Ceres, Inc. (Thousand Oaks, CA) for providing switchgrass feedstocks.

PY - 2011/12

Y1 - 2011/12

N2 - The objective of this work is to investigate the effects of cellulase loading and β-glucosidase supplementation on enzymatic hydrolysis of pretreated Dacotah switchgrass. To assess the difference among various pretreatment methods, the profiles of sugars and intermediates were determined for differently treated substrates. For all pretreatments, 72h glucan/xylan digestibilities increased sharply with enzyme loading up to 25mg protein/g-glucan, after which the response varied depending on the pretreatment method. For a fixed level of enzyme loading, dilute sulfuric acid (DA), SO 2, and Lime pretreatments exhibited higher digestibility than the soaking in aqueous ammonia (SAA) and ammonia fiber expansion (AFEX). Supplementation of Novozyme-188 to Spezyme-CP improved the 72h glucan digestibility only for the SAA treated samples. The effect of β-glucosidase supplementation was discernible only at the early phase of hydrolysis where accumulation of cellobiose and oligomers is significant. Addition of β-glucosidase increased the xylan digestibility of alkaline treated samples due to the β-xylosidase activity present in Novozyme-188.

AB - The objective of this work is to investigate the effects of cellulase loading and β-glucosidase supplementation on enzymatic hydrolysis of pretreated Dacotah switchgrass. To assess the difference among various pretreatment methods, the profiles of sugars and intermediates were determined for differently treated substrates. For all pretreatments, 72h glucan/xylan digestibilities increased sharply with enzyme loading up to 25mg protein/g-glucan, after which the response varied depending on the pretreatment method. For a fixed level of enzyme loading, dilute sulfuric acid (DA), SO 2, and Lime pretreatments exhibited higher digestibility than the soaking in aqueous ammonia (SAA) and ammonia fiber expansion (AFEX). Supplementation of Novozyme-188 to Spezyme-CP improved the 72h glucan digestibility only for the SAA treated samples. The effect of β-glucosidase supplementation was discernible only at the early phase of hydrolysis where accumulation of cellobiose and oligomers is significant. Addition of β-glucosidase increased the xylan digestibility of alkaline treated samples due to the β-xylosidase activity present in Novozyme-188.

KW - Cellulase

KW - Digestibility

KW - Pretreatment

KW - Switchgrass

KW - β-Glucosidase

UR - http://www.scopus.com/inward/record.url?scp=82755194815&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82755194815&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2011.03.085

DO - 10.1016/j.biortech.2011.03.085

M3 - Article

C2 - 21507624

AN - SCOPUS:82755194815

SN - 0960-8524

VL - 102

SP - 11115

EP - 11120

JO - Bioresource Technology

JF - Bioresource Technology

IS - 24

ER -

Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologies

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this