Effects of indomethacin, luteinizing hormone (LH), prostaglandin E2 (PGE2), trilostane, mifepristone, ethamoxytriphetol (MER-25) on secretion of prostaglandin E (PGE), prostaglandin F2α (PGF2α) and progesterone by ovine corpora lutea of pregnancy or the estrous cycle

L. Kim; Y. S. Weems; P. J. Bridges; B. R. LeaMaster; L. Ching; D. L. Vincent; C. W. Weems

doi:10.1016/S0090-6980(01)00097-1

Effects of indomethacin, luteinizing hormone (LH), prostaglandin E₂ (PGE₂), trilostane, mifepristone, ethamoxytriphetol (MER-25) on secretion of prostaglandin E (PGE), prostaglandin F_2α (PGF_2α) and progesterone by ovine corpora lutea of pregnancy or the estrous cycle

L. Kim, Y. S. Weems, P. J. Bridges, B. R. LeaMaster, L. Ching, D. L. Vincent, C. W. Weems

Animal and Food Sciences

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Two experiments were conducted to determine the luteotropin of pregnancy in sheep and to examine autocrine and paracrine roles of progesterone and estradiol-17 β on progesterone secretion by the ovine corpus luteum (CL). Secretion of progesterone per unit mass by day-8 or day-11 CL of the estrous cycle was similar to day-90 CL of pregnancy (P ≥ 0.05). In experiment 1, secretion of progesterone in vitro by slices of CL from ewes on day-8 of the estrous cycle was increased (P ≤ 0.05) by LH or PGE₂. Secretion of progesterone in vitro by CL slices from day-90 pregnant ewes was not affected by LH (P ≥ 0.05) while PGE₂ increased (P ≤ 0.05) secretion of progesterone. Day 8 ovine CL of the estrous cycle did not secrete (P ≥ 0.05) detectable quantities of PGF_2α or PGE while day-90 ovine CL of pregnancy secreted PGE (P ≤ 0.05) but not PGF_2α. Secretion of progesterone and PGE in vitro by day-90 CL of pregnancy was decreased (P ≤ 0.05) by indomethacin. The addition of PGE₂, but not LH, in combination with indomethacin overcame the decreases in progesterone by indomethacin (P ≤ 0.05). In experiment 2, secretion of progesterone in vitro by day-11 CL of the estrous cycle was increased at 4-h (P ≤ 0.05) in the absence of treatments. Both day-11 CL of the estrous cycle and day-90 CL of pregnancy secreted detectable quantities of PGE and PGF_2α (P ≤ 0.05). In experiment 1, PGF_2α secretion by day-8 CL of the estrous cycle and day-90 ovine CL of pregnancy was undetectable, but was detectable in experiment 2 by day-90 CL. Day 90 ovine CL of pregnancy also secreted more PGE than day-11 CL of the estrous cycle (P ≤ 0.05), whereas day-8 CL of the estrous cycle did not secrete detectable quantities of PGE (P ≥ 0.05). Trilostane, mifepristone, or MER-25 did not affect secretion of progesterone, PGE, or PGF_2α by day-11 CL of the estrous cycle or day-90 CL of pregnancy (P ≥ 0.05). It is concluded that PGE₂, not LH, is the luteotropin at day-90 of pregnancy in sheep and that progesterone does not modify the response to luteotropins. Thus, we found no evidence for an autocrine or paracrine role for progesterone or estradiol-17 36 on luteal secretion of progesterone, PGE or PGF_2α.

Original language	English
Pages (from-to)	189-203
Number of pages	15
Journal	Prostaglandins and Other Lipid Mediators
Volume	63
Issue number	4
DOIs	https://doi.org/10.1016/S0090-6980(01)00097-1
State	Published - 2001

Bibliographical note

Funding Information:
The authors gratefully acknowledge the assistance of Mr. Alan Umaki and Armand Unabia, Waialee Livestock Research Farm for care, estrous detection, and mating of sheep used in these studies. The antiserum for the progesterone assay was kindly supplied by Dr. R.L. Butcher, West Virginia University, antisera for PGF 2α assays was kindly provided by Dr. L. Levine, Brandeis University, Waltham, MA and antisera for PGE assays was kindly supplied by Dr. N. Mason, Lily Research Laboratories, Indianapolis, IN. Also, the authors acknowledge the support of Mr. Bruce Robinson, Niihau Ranch. The authors also want to thank Mr. Eric Johnson, Hawaii Mega-Cor Inc., Honolulu, HI for providing surgical supplies. The authors want to thank Mr. Wayne Toma for assistance with statistical analysis.

ASJC Scopus subject areas

Biochemistry
Physiology
Pharmacology
Cell Biology

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Kim, L., Weems, Y. S., Bridges, P. J., LeaMaster, B. R., Ching, L., Vincent, D. L., & Weems, C. W. (2001). Effects of indomethacin, luteinizing hormone (LH), prostaglandin E₂ (PGE₂), trilostane, mifepristone, ethamoxytriphetol (MER-25) on secretion of prostaglandin E (PGE), prostaglandin F_2α (PGF_2α) and progesterone by ovine corpora lutea of pregnancy or the estrous cycle. Prostaglandins and Other Lipid Mediators, 63(4), 189-203. https://doi.org/10.1016/S0090-6980(01)00097-1

Kim, L. ; Weems, Y. S. ; Bridges, P. J. et al. / Effects of indomethacin, luteinizing hormone (LH), prostaglandin E₂ (PGE₂), trilostane, mifepristone, ethamoxytriphetol (MER-25) on secretion of prostaglandin E (PGE), prostaglandin F_2α (PGF_2α) and progesterone by ovine corpora lutea of pregnancy or the estrous cycle. In: Prostaglandins and Other Lipid Mediators. 2001 ; Vol. 63, No. 4. pp. 189-203.

@article{2c90f01f2f1641b69f225950760bfa58,

title = "Effects of indomethacin, luteinizing hormone (LH), prostaglandin E2 (PGE2), trilostane, mifepristone, ethamoxytriphetol (MER-25) on secretion of prostaglandin E (PGE), prostaglandin F2α (PGF2α) and progesterone by ovine corpora lutea of pregnancy or the estrous cycle",

abstract = "Two experiments were conducted to determine the luteotropin of pregnancy in sheep and to examine autocrine and paracrine roles of progesterone and estradiol-17 β on progesterone secretion by the ovine corpus luteum (CL). Secretion of progesterone per unit mass by day-8 or day-11 CL of the estrous cycle was similar to day-90 CL of pregnancy (P ≥ 0.05). In experiment 1, secretion of progesterone in vitro by slices of CL from ewes on day-8 of the estrous cycle was increased (P ≤ 0.05) by LH or PGE2. Secretion of progesterone in vitro by CL slices from day-90 pregnant ewes was not affected by LH (P ≥ 0.05) while PGE2 increased (P ≤ 0.05) secretion of progesterone. Day 8 ovine CL of the estrous cycle did not secrete (P ≥ 0.05) detectable quantities of PGF2α or PGE while day-90 ovine CL of pregnancy secreted PGE (P ≤ 0.05) but not PGF2α. Secretion of progesterone and PGE in vitro by day-90 CL of pregnancy was decreased (P ≤ 0.05) by indomethacin. The addition of PGE2, but not LH, in combination with indomethacin overcame the decreases in progesterone by indomethacin (P ≤ 0.05). In experiment 2, secretion of progesterone in vitro by day-11 CL of the estrous cycle was increased at 4-h (P ≤ 0.05) in the absence of treatments. Both day-11 CL of the estrous cycle and day-90 CL of pregnancy secreted detectable quantities of PGE and PGF2α (P ≤ 0.05). In experiment 1, PGF2α secretion by day-8 CL of the estrous cycle and day-90 ovine CL of pregnancy was undetectable, but was detectable in experiment 2 by day-90 CL. Day 90 ovine CL of pregnancy also secreted more PGE than day-11 CL of the estrous cycle (P ≤ 0.05), whereas day-8 CL of the estrous cycle did not secrete detectable quantities of PGE (P ≥ 0.05). Trilostane, mifepristone, or MER-25 did not affect secretion of progesterone, PGE, or PGF2α by day-11 CL of the estrous cycle or day-90 CL of pregnancy (P ≥ 0.05). It is concluded that PGE2, not LH, is the luteotropin at day-90 of pregnancy in sheep and that progesterone does not modify the response to luteotropins. Thus, we found no evidence for an autocrine or paracrine role for progesterone or estradiol-17 36 on luteal secretion of progesterone, PGE or PGF2α.",

author = "L. Kim and Weems, {Y. S.} and Bridges, {P. J.} and LeaMaster, {B. R.} and L. Ching and Vincent, {D. L.} and Weems, {C. W.}",

note = "Funding Information: The authors gratefully acknowledge the assistance of Mr. Alan Umaki and Armand Unabia, Waialee Livestock Research Farm for care, estrous detection, and mating of sheep used in these studies. The antiserum for the progesterone assay was kindly supplied by Dr. R.L. Butcher, West Virginia University, antisera for PGF 2α assays was kindly provided by Dr. L. Levine, Brandeis University, Waltham, MA and antisera for PGE assays was kindly supplied by Dr. N. Mason, Lily Research Laboratories, Indianapolis, IN. Also, the authors acknowledge the support of Mr. Bruce Robinson, Niihau Ranch. The authors also want to thank Mr. Eric Johnson, Hawaii Mega-Cor Inc., Honolulu, HI for providing surgical supplies. The authors want to thank Mr. Wayne Toma for assistance with statistical analysis. ",

year = "2001",

doi = "10.1016/S0090-6980(01)00097-1",

language = "English",

volume = "63",

pages = "189--203",

number = "4",

}

Kim, L, Weems, YS, Bridges, PJ, LeaMaster, BR, Ching, L, Vincent, DL & Weems, CW 2001, 'Effects of indomethacin, luteinizing hormone (LH), prostaglandin E₂ (PGE₂), trilostane, mifepristone, ethamoxytriphetol (MER-25) on secretion of prostaglandin E (PGE), prostaglandin F_2α (PGF_2α) and progesterone by ovine corpora lutea of pregnancy or the estrous cycle', Prostaglandins and Other Lipid Mediators, vol. 63, no. 4, pp. 189-203. https://doi.org/10.1016/S0090-6980(01)00097-1

Effects of indomethacin, luteinizing hormone (LH), prostaglandin E₂ (PGE₂), trilostane, mifepristone, ethamoxytriphetol (MER-25) on secretion of prostaglandin E (PGE), prostaglandin F_2α (PGF_2α) and progesterone by ovine corpora lutea of pregnancy or the estrous cycle. / Kim, L.; Weems, Y. S.; Bridges, P. J. et al.
In: Prostaglandins and Other Lipid Mediators, Vol. 63, No. 4, 2001, p. 189-203.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Effects of indomethacin, luteinizing hormone (LH), prostaglandin E2 (PGE2), trilostane, mifepristone, ethamoxytriphetol (MER-25) on secretion of prostaglandin E (PGE), prostaglandin F2α (PGF2α) and progesterone by ovine corpora lutea of pregnancy or the estrous cycle

AU - Kim, L.

AU - Weems, Y. S.

AU - Bridges, P. J.

AU - LeaMaster, B. R.

AU - Ching, L.

AU - Vincent, D. L.

AU - Weems, C. W.

N1 - Funding Information: The authors gratefully acknowledge the assistance of Mr. Alan Umaki and Armand Unabia, Waialee Livestock Research Farm for care, estrous detection, and mating of sheep used in these studies. The antiserum for the progesterone assay was kindly supplied by Dr. R.L. Butcher, West Virginia University, antisera for PGF 2α assays was kindly provided by Dr. L. Levine, Brandeis University, Waltham, MA and antisera for PGE assays was kindly supplied by Dr. N. Mason, Lily Research Laboratories, Indianapolis, IN. Also, the authors acknowledge the support of Mr. Bruce Robinson, Niihau Ranch. The authors also want to thank Mr. Eric Johnson, Hawaii Mega-Cor Inc., Honolulu, HI for providing surgical supplies. The authors want to thank Mr. Wayne Toma for assistance with statistical analysis.

PY - 2001

Y1 - 2001

N2 - Two experiments were conducted to determine the luteotropin of pregnancy in sheep and to examine autocrine and paracrine roles of progesterone and estradiol-17 β on progesterone secretion by the ovine corpus luteum (CL). Secretion of progesterone per unit mass by day-8 or day-11 CL of the estrous cycle was similar to day-90 CL of pregnancy (P ≥ 0.05). In experiment 1, secretion of progesterone in vitro by slices of CL from ewes on day-8 of the estrous cycle was increased (P ≤ 0.05) by LH or PGE2. Secretion of progesterone in vitro by CL slices from day-90 pregnant ewes was not affected by LH (P ≥ 0.05) while PGE2 increased (P ≤ 0.05) secretion of progesterone. Day 8 ovine CL of the estrous cycle did not secrete (P ≥ 0.05) detectable quantities of PGF2α or PGE while day-90 ovine CL of pregnancy secreted PGE (P ≤ 0.05) but not PGF2α. Secretion of progesterone and PGE in vitro by day-90 CL of pregnancy was decreased (P ≤ 0.05) by indomethacin. The addition of PGE2, but not LH, in combination with indomethacin overcame the decreases in progesterone by indomethacin (P ≤ 0.05). In experiment 2, secretion of progesterone in vitro by day-11 CL of the estrous cycle was increased at 4-h (P ≤ 0.05) in the absence of treatments. Both day-11 CL of the estrous cycle and day-90 CL of pregnancy secreted detectable quantities of PGE and PGF2α (P ≤ 0.05). In experiment 1, PGF2α secretion by day-8 CL of the estrous cycle and day-90 ovine CL of pregnancy was undetectable, but was detectable in experiment 2 by day-90 CL. Day 90 ovine CL of pregnancy also secreted more PGE than day-11 CL of the estrous cycle (P ≤ 0.05), whereas day-8 CL of the estrous cycle did not secrete detectable quantities of PGE (P ≥ 0.05). Trilostane, mifepristone, or MER-25 did not affect secretion of progesterone, PGE, or PGF2α by day-11 CL of the estrous cycle or day-90 CL of pregnancy (P ≥ 0.05). It is concluded that PGE2, not LH, is the luteotropin at day-90 of pregnancy in sheep and that progesterone does not modify the response to luteotropins. Thus, we found no evidence for an autocrine or paracrine role for progesterone or estradiol-17 36 on luteal secretion of progesterone, PGE or PGF2α.

AB - Two experiments were conducted to determine the luteotropin of pregnancy in sheep and to examine autocrine and paracrine roles of progesterone and estradiol-17 β on progesterone secretion by the ovine corpus luteum (CL). Secretion of progesterone per unit mass by day-8 or day-11 CL of the estrous cycle was similar to day-90 CL of pregnancy (P ≥ 0.05). In experiment 1, secretion of progesterone in vitro by slices of CL from ewes on day-8 of the estrous cycle was increased (P ≤ 0.05) by LH or PGE2. Secretion of progesterone in vitro by CL slices from day-90 pregnant ewes was not affected by LH (P ≥ 0.05) while PGE2 increased (P ≤ 0.05) secretion of progesterone. Day 8 ovine CL of the estrous cycle did not secrete (P ≥ 0.05) detectable quantities of PGF2α or PGE while day-90 ovine CL of pregnancy secreted PGE (P ≤ 0.05) but not PGF2α. Secretion of progesterone and PGE in vitro by day-90 CL of pregnancy was decreased (P ≤ 0.05) by indomethacin. The addition of PGE2, but not LH, in combination with indomethacin overcame the decreases in progesterone by indomethacin (P ≤ 0.05). In experiment 2, secretion of progesterone in vitro by day-11 CL of the estrous cycle was increased at 4-h (P ≤ 0.05) in the absence of treatments. Both day-11 CL of the estrous cycle and day-90 CL of pregnancy secreted detectable quantities of PGE and PGF2α (P ≤ 0.05). In experiment 1, PGF2α secretion by day-8 CL of the estrous cycle and day-90 ovine CL of pregnancy was undetectable, but was detectable in experiment 2 by day-90 CL. Day 90 ovine CL of pregnancy also secreted more PGE than day-11 CL of the estrous cycle (P ≤ 0.05), whereas day-8 CL of the estrous cycle did not secrete detectable quantities of PGE (P ≥ 0.05). Trilostane, mifepristone, or MER-25 did not affect secretion of progesterone, PGE, or PGF2α by day-11 CL of the estrous cycle or day-90 CL of pregnancy (P ≥ 0.05). It is concluded that PGE2, not LH, is the luteotropin at day-90 of pregnancy in sheep and that progesterone does not modify the response to luteotropins. Thus, we found no evidence for an autocrine or paracrine role for progesterone or estradiol-17 36 on luteal secretion of progesterone, PGE or PGF2α.

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Kim L, Weems YS, Bridges PJ, LeaMaster BR, Ching L, Vincent DL et al. Effects of indomethacin, luteinizing hormone (LH), prostaglandin E₂ (PGE₂), trilostane, mifepristone, ethamoxytriphetol (MER-25) on secretion of prostaglandin E (PGE), prostaglandin F_2α (PGF_2α) and progesterone by ovine corpora lutea of pregnancy or the estrous cycle. Prostaglandins and Other Lipid Mediators. 2001;63(4):189-203. doi: 10.1016/S0090-6980(01)00097-1