Effects of insulin-like peptide 7 in Bemisia tabaci MED on tomato chlorosis virus transmission

Hui Nan Xu, Zhan Hong Zhang, Zhuo Zhang, Jing Peng, Yang Gao, Kai Long Li, Jianbin Chen, Jiao Du, Shuo Yan, De Yong Zhang, Xu Guo Zhou, Xiao Bin Shi, Yong Liu

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Tomato chlorosis virus (ToCV) is a semi-persistent plant virus that is primarily transmitted by the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae). It causes a serious disease that lowers tomato yield. Insulin-like peptide (ILP), an insulin homolog, regulates trehalose metabolism in a variety of insects. In a previous study, we discovered that trehalose metabolism is required for whiteflies to transmit ToCV effectively. Furthermore, transcriptome sequencing revealed that the BtILP7 gene was highly expressed in B. tabaci infected with ToCV. Therefore, the whitefly ILP7 gene may facilitate the transmission of ToCV and be an attractive target for the control of whiteflies and subsequently ToCV. RESULTS: The ToCV content in B. tabaci MED was found to be correlated with BtILP7 gene expression. Subsequent RNA interference (RNAi) of the BtILP7 gene had a significant impact on B. tabaci MED's trehalose metabolism and reproductive capacity, as well as ability to transmit ToCV. CONCLUSIONS: These results indicate that the BtILP7 gene was closely related to ToCV transmission by regulating trehalose metabolism and reproduction behavior, thus providing a secure and environmentally friendly management strategy for the control of whiteflies and ToCV-caused disease.

Original languageEnglish
Pages (from-to)1508-1517
Number of pages10
JournalPest Management Science
Volume79
Issue number4
DOIs
StatePublished - Apr 2023

Bibliographical note

Funding Information:
The authors thank Prof. Tao Zhou, China Agricultural University, for providing clones of ToCV inocula. This work was supported by the National Natural Science Foundation of China (32030088, 32272535, 31972242, and 32072383), the Agriculture Research System of China (CARS‐23‐D‐02), and the National Key Research and Development Project (2018YFE0112600).

Publisher Copyright:
© 2022 Society of Chemical Industry.

Keywords

  • Bemisia tabaci MED
  • RNA interference
  • insulin-like peptide 7
  • tomato chlorosis virus
  • trehalose metabolism

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

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