Effects of melatonin on 2-deoxy-[1-14C]glucose uptake within rat suprachiasmatic nucleus

V. M. Cassone, M. H. Roberts, R. Y. Moore

Research output: Contribution to journalArticlepeer-review

104 Scopus citations


Previously, we have demonstrated that metabolic activity, shown by autoradiographic determination of 2-deoxy-[1-14C]glucose (2-DG) uptake, within the rat hypothalamic suprachiasmatic nuclei (SCN) was inhibited by subcutaneous injection of 1 mg/kg melatonin. To determine whether this effect was specific to a particular time of day, the effects of melatonin on 2-DG uptake were studied in several hypothalamic areas, including the SCN, supraoptic nuclei (SON), lateral hypothalamic area (LHA), and anterior hypothalamic area (AHA) every 4 h throughout the circadian day. In a second experiment, the effects of different melatonin doses were studied at the time of day at which melatonin had its maximal effect to determine the dose-response relationship of melatonin-induced inhibition of SCN 2-DG uptake. The data indicate that melatonin inhibited 2-DG uptake in the SCN alone at one time of day, primarily at circadian time (CT) 6 and CT10, 2-6 h before subjective dusk, and secondarily at CT22, just before subjective dawn. This effect was dose dependent with a 50% effective dose of 1.49 ± 2.30 μg/kg. The temporal and dose-response characteristics of these effects are similar to those characterizing the entraining effects of melatonin on circadian patterns of locomotion and drinking.

Original languageEnglish
Pages (from-to)24/2
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Issue number2
StatePublished - 1988

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


Dive into the research topics of 'Effects of melatonin on 2-deoxy-[1-14C]glucose uptake within rat suprachiasmatic nucleus'. Together they form a unique fingerprint.

Cite this