Abstract
The microbial activity of the inoculum (liquid anaerobic digestion effluent) was altered by autoclaving part of the effluent to study the effect of feedstock to active effluent ratio (F/Ea, 2.2-6.6) and the feedstock to total effluent ratio (F/Et, 2.2 and 4.4) on reactor performance in solid state anaerobic digestion (SS-AD) of corn stover. When the F/Ea ratio was increased from 2.2 to 6.6, methane yield was not significantly reduced; however, reactors became acidified when the F/Et ratio was increased from 2.2 to 4.4. It was concluded that F/Et had a greater effect on methane yields than F/Ea for the range studied in this paper. As analyzed by denaturing gradient gel electrophoresis using PCR amplified 16S rRNA genes, the microbial community underwent dynamic shifts under acidified conditions over 38. days of SS-AD. These shifts reflected the acclimation, both adaptive selection and diversification, of the initial inoculated microbial consortia.
| Original language | English |
|---|---|
| Pages (from-to) | 188-196 |
| Number of pages | 9 |
| Journal | Bioresource Technology |
| Volume | 157 |
| DOIs | |
| State | Published - Apr 2014 |
Bibliographical note
Funding Information:This project was funded by the USDA NIFA Biomass Research and Development Initiative Program (Award No. 2012-10008-20302 ) and USDA NIFA Hatch Program . The authors would like to thank Mrs. Mary Wicks (Department of Food, Agricultural and Biological Engineering, OSU) for reading through the manuscript and providing useful suggestions.
Keywords
- Anaerobic digestion
- Biogas
- Dry fermentation
- Inoculum
- Microbial community
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal
