Effects of oxidative and nitrosative stress in brain on p53 proapoptotic protein in amnestic mild cognitive impairment and Alzheimer disease

Giovanna Cenini, Rukhsana Sultana, Maurizio Memo, D. Allan Butterfield

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Many studies reported that oxidative and nitrosative stress might be important for the pathogenesis of Alzheimer's disease (AD) beginning with arguably the earliest stage of AD, i.e., as mild cognitive impairment (MCI). p53 is a proapoptotic protein that plays an important role in neuronal death, a process involved in many neurodegenerative disorders. Moreover, p53 plays a key role in the oxidative stress-dependent apoptosis. We demonstrated previously that p53 levels in brain were significantly higher in MCI and AD IPL (inferior parietal lobule) compared to control brains. In addition, we showed that in AD IPL, but not in MCI, HNE, a lipid peroxidation product, was significantly bound to p53 protein. In this report, we studied by means of immunoprecipitation analysis, the levels of markers of protein oxidation, 3-nitrotyrosine (3-NT) and protein carbonyls, in p53 in a specific region of the cerebral cortex, namely the inferior parietal lobule, in MCI and AD compared to control brains. The focus of these studies was to measure the oxidation and nitration status of this important proapoptotic protein, consistent with the hypothesis that oxidative modification of p53 could be involved in the neuronal loss observed in neurodegenerative conditions.

Original languageEnglish
Pages (from-to)81-85
Number of pages5
JournalFree Radical Biology and Medicine
Volume45
Issue number1
DOIs
StatePublished - Jul 1 2008

Bibliographical note

Funding Information:
This research was supported in part by NIH grants to D.A.B. [AG-05119, AG10836; AG-029839].

Keywords

  • 3-Nitrotyrosine
  • Alzheimer's disease (AD)
  • Apoptosis
  • Mild cognitive impairment (MCI)
  • Oxidative stress
  • Protein carbonyl
  • p53

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Fingerprint

Dive into the research topics of 'Effects of oxidative and nitrosative stress in brain on p53 proapoptotic protein in amnestic mild cognitive impairment and Alzheimer disease'. Together they form a unique fingerprint.

Cite this