TY - JOUR
T1 - Effects of Polyunsaturated Free Fatty Acids and Esterified Linoleoyl Derivatives on Oxygen Consumption and C6 Aldehyde Formation with Soybean Seed Homogenates
AU - Zhuang, Hong
AU - Hildebrand, David F.
AU - Andersen, Roger A.
AU - Hamilton-Kemp, Thomas R.
PY - 1991/8/1
Y1 - 1991/8/1
N2 - We investigated the effects of the form of fatty acid substrate with specific lipoxygenase (LOX) isozymes on O2 uptake and C6 compound formation using a soybean seed model system at pH 6.8. in most cases lipid-dependent O2 consumption with different unsaturated free fatty acids and 18:2 derivatives was qualitatively similar to C6 aldehyde formation. LOX 1 activity was higher with arachidonic acid and linolenic acid than linoleic acid, but with LOX 1 and/or LOX 2, linoleic acid yielded the highest C6 aldehyde production. the O2 uptake and C6 aldehyde production varied considerably among the esterified forms of linoleic acid. LOX 1 was relatively more effective in the lipid-dependent O2 uptake of free fatty acids compared to esterified linoleoyl derivatives, whereas LOX 2 or 3 was relatively more effective with esterified linoleic acid than LOX 1. LOX 2 gave the highest C6 aldehyde production with all substrates except with linoleoyl coenzyme A, where LOX 1 was most effective. the C6 aldehyde formation was reduced with all substrates when LOX 3 was present in the seed homogenates.
AB - We investigated the effects of the form of fatty acid substrate with specific lipoxygenase (LOX) isozymes on O2 uptake and C6 compound formation using a soybean seed model system at pH 6.8. in most cases lipid-dependent O2 consumption with different unsaturated free fatty acids and 18:2 derivatives was qualitatively similar to C6 aldehyde formation. LOX 1 activity was higher with arachidonic acid and linolenic acid than linoleic acid, but with LOX 1 and/or LOX 2, linoleic acid yielded the highest C6 aldehyde production. the O2 uptake and C6 aldehyde production varied considerably among the esterified forms of linoleic acid. LOX 1 was relatively more effective in the lipid-dependent O2 uptake of free fatty acids compared to esterified linoleoyl derivatives, whereas LOX 2 or 3 was relatively more effective with esterified linoleic acid than LOX 1. LOX 2 gave the highest C6 aldehyde production with all substrates except with linoleoyl coenzyme A, where LOX 1 was most effective. the C6 aldehyde formation was reduced with all substrates when LOX 3 was present in the seed homogenates.
UR - http://www.scopus.com/inward/record.url?scp=33751499772&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33751499772&partnerID=8YFLogxK
U2 - 10.1021/jf00008a001
DO - 10.1021/jf00008a001
M3 - Article
AN - SCOPUS:33751499772
SN - 0021-8561
VL - 39
SP - 1357
EP - 1364
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 8
ER -