Abstract
Temperature and light affected desaturation of linoleic acid (18:2) to linolenic acid (18:3) at the sn-1 and sn-2 positions of specific lipids in an independent manner in callus tissue of Arabidopsis thaliania. The prokaryotic pathway of desaturation predominated in tissue grown at 28° while at 18° the eukaryotic pathway predominated. The effects of the fadD mutation were diminished by continued growth in darkness. 4-Chloro-5-dimethylamino-2-phenyl-3(2H)-pyridazinone, a compound thought to reduce desaturation of 18:2 by the prokaryotic pathway, caused a reduction in a specific polypeptide. This same polypeptide was reduced in a mutant of Arabidopsis (fadD) that has genetically reduced amounts of 18:3. The correlation between reduced levels of this polypeptide and reduced levels of 18:3 suggest an involvement of this polypeptide in fatty acid desaturation.
Original language | English |
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Pages (from-to) | 1447-1453 |
Number of pages | 7 |
Journal | Phytochemistry |
Volume | 29 |
Issue number | 5 |
DOIs | |
State | Published - 1990 |
Keywords
- Arabidopsis thaliana
- Cruciferae
- environmental effects
- lipid synthesis
- mutants
- polyunsaturated fatty acids
- tissue culture.
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Plant Science
- Horticulture