Effects of temperature, light and a chemical modulator on linolenate biosynthesis in mutant and wild type Arabidopsis calli

Jeffrey A. Brockman, Helen A. Norman, David F. Hildebrand

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Temperature and light affected desaturation of linoleic acid (18:2) to linolenic acid (18:3) at the sn-1 and sn-2 positions of specific lipids in an independent manner in callus tissue of Arabidopsis thaliania. The prokaryotic pathway of desaturation predominated in tissue grown at 28° while at 18° the eukaryotic pathway predominated. The effects of the fadD mutation were diminished by continued growth in darkness. 4-Chloro-5-dimethylamino-2-phenyl-3(2H)-pyridazinone, a compound thought to reduce desaturation of 18:2 by the prokaryotic pathway, caused a reduction in a specific polypeptide. This same polypeptide was reduced in a mutant of Arabidopsis (fadD) that has genetically reduced amounts of 18:3. The correlation between reduced levels of this polypeptide and reduced levels of 18:3 suggest an involvement of this polypeptide in fatty acid desaturation.

Original languageEnglish
Pages (from-to)1447-1453
Number of pages7
JournalPhytochemistry
Volume29
Issue number5
DOIs
StatePublished - 1990

Keywords

  • Arabidopsis thaliana
  • Cruciferae
  • environmental effects
  • lipid synthesis
  • mutants
  • polyunsaturated fatty acids
  • tissue culture.

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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