Effects of the intermediate retinoid metabolite retinal on the pattern of the dental lamina in vitro

Jan E. Kronmiller, Cynthia S. Beeman, Kevin Kwiecien, Treven Rollins

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Retinoids have important roles in pattern formation during embryonic development and might act as endogenous morphogens. They are necessary for normal odontogenesis and excess retinol alters the pattern of odontogenesis producing supernumerary buds of the dental lamina in the diastema region of the mouse mandible. Because the metabolism of retinoids in the developing mandible is unknown, the effects of retinal (an intermediate metabolite in the local conversion of retinol to retinoic acid) on the patterning of odontogenesis were examined. Retinal produces supernumerary buds and enhanced epithelial proliferation in day-9 mandibles in vitro. The endogenous levels of retinal in the mandible at the time of initiation of odontogenesis were also measured by high-performance liquid chromatography. Retinal was detected only at day 10 and not at later stages of development. Local metabolism of this intermediate retinoid may be a rate-determining step in the production of active retinoid metabolites that may control the pattern of the dentition, which is established at the time of the appearance of the dental lamina at embryonic day 12.

Original languageEnglish
Pages (from-to)839-845
Number of pages7
JournalArchives of Oral Biology
Issue number10
StatePublished - Oct 1994

Bibliographical note

Funding Information:
AcknoM,ledgemenr~This work was supported in part by USPHS Grant No. DE00286-03 (JEK); and USPHS Grant No. DE10839-01 (JEK).


  • HPLC
  • odontogenesis
  • pattern formation
  • retinal
  • retinoids

ASJC Scopus subject areas

  • Otorhinolaryngology
  • General Dentistry
  • Cell Biology


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