TY - JOUR
T1 - Effects of UVB-induced oxidative stress on protein expression and specific protein oxidation in normal human epithelial keratinocytes
T2 - A proteomic approach
AU - Perluigi, Marzia
AU - Di Domenico, Fabio
AU - Blarzino, Carla
AU - Foppoli, Cesira
AU - Cini, Chiara
AU - Giorgi, Alessandra
AU - Grillo, Caterina
AU - De Marco, Federico
AU - Butterfield, David A.
AU - Schininà, Maria E.
AU - Coccia, Raffaella
N1 - Funding Information:
This work was partially supported by grants of the Italian Ministry of Health, the Italian Ministry of Foreign affairs and by the National Research Council. We wish to thank Dr Daniela Di Sciullo and Mr Vincenzo Peresempio for their precious and skilled technical work and ms Tania Merlino for her linguistic revision of the manuscript.
PY - 2010/3/18
Y1 - 2010/3/18
N2 - Background: The UVB component of solar ultraviolet irradiation is one of the major risk factors for the development of skin cancer in humans. UVB exposure elicits an increased generation of reactive oxygen species (ROS), which are responsible for oxidative damage to proteins, DNA, RNA and lipids. In order to examine the biological impact of UVB irradiation on skin cells, we used a parallel proteomics approach to analyze the protein expression profile and to identify oxidatively modified proteins in normal human epithelial keratinocytes.Results: The expression levels of fifteen proteins - involved in maintaining the cytoskeleton integrity, removal of damaged proteins and heat shock response - were differentially regulated in UVB-exposed cells, indicating that an appropriate response is developed in order to counteract/neutralize the toxic effects of UVB-raised ROS. On the other side, the redox proteomics approach revealed that seven proteins - involved in cellular adhesion, cell-cell interaction and protein folding - were selectively oxidized.Conclusions: Despite a wide and well orchestrated cellular response, a relevant oxidation of specific proteins concomitantly occurs in UVB-irradiated human epithelial Keratinocytes. These modified (i.e. likely dysfunctional) proteins might result in cell homeostasis impairment and therefore eventually promote cellular degeneration, senescence or carcinogenesis.
AB - Background: The UVB component of solar ultraviolet irradiation is one of the major risk factors for the development of skin cancer in humans. UVB exposure elicits an increased generation of reactive oxygen species (ROS), which are responsible for oxidative damage to proteins, DNA, RNA and lipids. In order to examine the biological impact of UVB irradiation on skin cells, we used a parallel proteomics approach to analyze the protein expression profile and to identify oxidatively modified proteins in normal human epithelial keratinocytes.Results: The expression levels of fifteen proteins - involved in maintaining the cytoskeleton integrity, removal of damaged proteins and heat shock response - were differentially regulated in UVB-exposed cells, indicating that an appropriate response is developed in order to counteract/neutralize the toxic effects of UVB-raised ROS. On the other side, the redox proteomics approach revealed that seven proteins - involved in cellular adhesion, cell-cell interaction and protein folding - were selectively oxidized.Conclusions: Despite a wide and well orchestrated cellular response, a relevant oxidation of specific proteins concomitantly occurs in UVB-irradiated human epithelial Keratinocytes. These modified (i.e. likely dysfunctional) proteins might result in cell homeostasis impairment and therefore eventually promote cellular degeneration, senescence or carcinogenesis.
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U2 - 10.1186/1477-5956-8-13
DO - 10.1186/1477-5956-8-13
M3 - Article
C2 - 20298559
AN - SCOPUS:77952922104
SN - 1477-5956
VL - 8
JO - Proteome Science
JF - Proteome Science
M1 - 13
ER -
