TY - GEN
T1 - Efficacy of select viability assays in multicellular tumor spheroid culture
AU - Coburn, Jonathan F.
AU - Patterson, Sarah E.
AU - Hauser, Anastasia K.
AU - Anderson, Kimberly W.
AU - Hilt, J. Zach
PY - 2014
Y1 - 2014
N2 - Multicellular tumor spheroids (MCTS) mimic the physiological state of tumors in vivo more accurately than do cell monolayers. Essential tumor characteristics such as hypoxia, drug resistance, and the extracellular matrix may collectively be included in cancer therapy screening through the use of this complex in vitro model. Attendant with the increased complexity of the MCTS model is increased difficulty of application, particularly in assaying MCTS viability. The objective of this study was to investigate the efficacy of the acid phosphatase, resazurin, and live/dead assays in A549 MCTS culture. Experiments were conducted to examine consistency and accuracy as well as usefulness for future hyperthermia and chemotherapy studies. The results presented herein contribute to the development and validation of assays necessary to the application of MCTS in cancer research.
AB - Multicellular tumor spheroids (MCTS) mimic the physiological state of tumors in vivo more accurately than do cell monolayers. Essential tumor characteristics such as hypoxia, drug resistance, and the extracellular matrix may collectively be included in cancer therapy screening through the use of this complex in vitro model. Attendant with the increased complexity of the MCTS model is increased difficulty of application, particularly in assaying MCTS viability. The objective of this study was to investigate the efficacy of the acid phosphatase, resazurin, and live/dead assays in A549 MCTS culture. Experiments were conducted to examine consistency and accuracy as well as usefulness for future hyperthermia and chemotherapy studies. The results presented herein contribute to the development and validation of assays necessary to the application of MCTS in cancer research.
UR - http://www.scopus.com/inward/record.url?scp=84958531809&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84958531809&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84958531809
T3 - Student Poster Sessions 2014 - Core Programming Area at the 2014 AIChE Annual Meeting
SP - 108
BT - Student Poster Sessions 2014 - Core Programming Area at the 2014 AIChE Annual Meeting
T2 - Student Poster Sessions 2014 - Core Programming Area at the 2014 AIChE Annual Meeting
Y2 - 16 November 2014 through 21 November 2014
ER -
