Egg White-Derived Antihypertensive Peptide IRW (Ile-Arg-Trp) Inhibits Angiotensin II-Stimulated Migration of Vascular Smooth Muscle Cells via Angiotensin Type i Receptor

Wang Liao; Hongbing Fan; Jianping Wu

doi:10.1021/acs.jafc.8b00483

Egg White-Derived Antihypertensive Peptide IRW (Ile-Arg-Trp) Inhibits Angiotensin II-Stimulated Migration of Vascular Smooth Muscle Cells via Angiotensin Type i Receptor

Wang Liao, Hongbing Fan, Jianping Wu

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Excessive proliferation, inflammation, oxidative stress, and migration induced by angiotensin II (Ang II), occurring in vascular smooth muscle cells (VSMCs) during vascular remodelling, are major pathogenesis of hypertension. Antihypertensive peptides derived from food proteins are promising alternatives in preventing/treating hypertension and associated complications. In addition to reducing high blood pressure in spontaneously hypertensive rats, egg white ovotransferrin-derived antihypertensive IRW (Ile-Arg-Trp) was shown to exert antiproliferative, antioxidant, and anti-inflammatory effects in A7r5 cells (a vascular smooth muscle cell line) against Ang II stimulation, further indicating its potential in retarding vascular remodelling. Since its regulatory role in migration of VSMC is unclear, the objective of this study was to evaluate the antimigrant activity of IRW in Ang II-stimulated A7r5 cells. It was found that IRW could downregulate matrix metallopeptidase 9 (MMP9) expression and inhibit migration of Ang II-stimulated A7r5 cells, which was associated with inactivation of p38/MAPK signaling. More importantly, the antimigrant activity of IRW in Ang II-stimulated A7r5 cells was dependent on angiotensin type I receptor (AT1R). Our study provided the first evidence that egg ovotransferrin-derived antihypertensive peptide IRW inhibited migration of VSMCs.

Original language	English
Pages (from-to)	5133-5138
Number of pages	6
Journal	Journal of Agricultural and Food Chemistry
Volume	66
Issue number	20
DOIs	https://doi.org/10.1021/acs.jafc.8b00483
State	Published - May 23 2018

Bibliographical note

Funding Information:
*E-mail: jwu3@ualberta.ca. Tel: 1-780-492-6885. Fax: 1-780-492-4265. Address: 4-10 Ag/For Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2P5. ORCID Wang Liao: 0000-0001-8319-2199 Jianping Wu: 0000-0003-2574-5191 Funding This work was funded by grants from the Alberta Livestock and Meat Agency (ALMA) and the Natural Sciences and Engineering Research Council (NSERC) to J.W. Notes The authors declare no competing financial interest.

Publisher Copyright:
© 2018 American Chemical Society.

Keywords

antihypertensive peptides
IRW
migration
ovotransferrin
vascular smooth muscle cell

ASJC Scopus subject areas

Chemistry (all)
Agricultural and Biological Sciences (all)

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10.1021/acs.jafc.8b00483

Cite this

@article{f562d7fed7a440e7a5e19bd9a8c9e65b,

title = "Egg White-Derived Antihypertensive Peptide IRW (Ile-Arg-Trp) Inhibits Angiotensin II-Stimulated Migration of Vascular Smooth Muscle Cells via Angiotensin Type i Receptor",

abstract = "Excessive proliferation, inflammation, oxidative stress, and migration induced by angiotensin II (Ang II), occurring in vascular smooth muscle cells (VSMCs) during vascular remodelling, are major pathogenesis of hypertension. Antihypertensive peptides derived from food proteins are promising alternatives in preventing/treating hypertension and associated complications. In addition to reducing high blood pressure in spontaneously hypertensive rats, egg white ovotransferrin-derived antihypertensive IRW (Ile-Arg-Trp) was shown to exert antiproliferative, antioxidant, and anti-inflammatory effects in A7r5 cells (a vascular smooth muscle cell line) against Ang II stimulation, further indicating its potential in retarding vascular remodelling. Since its regulatory role in migration of VSMC is unclear, the objective of this study was to evaluate the antimigrant activity of IRW in Ang II-stimulated A7r5 cells. It was found that IRW could downregulate matrix metallopeptidase 9 (MMP9) expression and inhibit migration of Ang II-stimulated A7r5 cells, which was associated with inactivation of p38/MAPK signaling. More importantly, the antimigrant activity of IRW in Ang II-stimulated A7r5 cells was dependent on angiotensin type I receptor (AT1R). Our study provided the first evidence that egg ovotransferrin-derived antihypertensive peptide IRW inhibited migration of VSMCs.",

keywords = "antihypertensive peptides, IRW, migration, ovotransferrin, vascular smooth muscle cell",

author = "Wang Liao and Hongbing Fan and Jianping Wu",

note = "Funding Information: *E-mail: jwu3@ualberta.ca. Tel: 1-780-492-6885. Fax: 1-780-492-4265. Address: 4-10 Ag/For Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2P5. ORCID Wang Liao: 0000-0001-8319-2199 Jianping Wu: 0000-0003-2574-5191 Funding This work was funded by grants from the Alberta Livestock and Meat Agency (ALMA) and the Natural Sciences and Engineering Research Council (NSERC) to J.W. Notes The authors declare no competing financial interest. Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

year = "2018",

month = may,

day = "23",

doi = "10.1021/acs.jafc.8b00483",

language = "English",

volume = "66",

pages = "5133--5138",

number = "20",

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Egg White-Derived Antihypertensive Peptide IRW (Ile-Arg-Trp) Inhibits Angiotensin II-Stimulated Migration of Vascular Smooth Muscle Cells via Angiotensin Type i Receptor. / Liao, Wang; Fan, Hongbing; Wu, Jianping.
In: Journal of Agricultural and Food Chemistry, Vol. 66, No. 20, 23.05.2018, p. 5133-5138.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Egg White-Derived Antihypertensive Peptide IRW (Ile-Arg-Trp) Inhibits Angiotensin II-Stimulated Migration of Vascular Smooth Muscle Cells via Angiotensin Type i Receptor

AU - Liao, Wang

AU - Fan, Hongbing

AU - Wu, Jianping

N1 - Funding Information: *E-mail: jwu3@ualberta.ca. Tel: 1-780-492-6885. Fax: 1-780-492-4265. Address: 4-10 Ag/For Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2P5. ORCID Wang Liao: 0000-0001-8319-2199 Jianping Wu: 0000-0003-2574-5191 Funding This work was funded by grants from the Alberta Livestock and Meat Agency (ALMA) and the Natural Sciences and Engineering Research Council (NSERC) to J.W. Notes The authors declare no competing financial interest. Publisher Copyright: © 2018 American Chemical Society.

PY - 2018/5/23

Y1 - 2018/5/23

N2 - Excessive proliferation, inflammation, oxidative stress, and migration induced by angiotensin II (Ang II), occurring in vascular smooth muscle cells (VSMCs) during vascular remodelling, are major pathogenesis of hypertension. Antihypertensive peptides derived from food proteins are promising alternatives in preventing/treating hypertension and associated complications. In addition to reducing high blood pressure in spontaneously hypertensive rats, egg white ovotransferrin-derived antihypertensive IRW (Ile-Arg-Trp) was shown to exert antiproliferative, antioxidant, and anti-inflammatory effects in A7r5 cells (a vascular smooth muscle cell line) against Ang II stimulation, further indicating its potential in retarding vascular remodelling. Since its regulatory role in migration of VSMC is unclear, the objective of this study was to evaluate the antimigrant activity of IRW in Ang II-stimulated A7r5 cells. It was found that IRW could downregulate matrix metallopeptidase 9 (MMP9) expression and inhibit migration of Ang II-stimulated A7r5 cells, which was associated with inactivation of p38/MAPK signaling. More importantly, the antimigrant activity of IRW in Ang II-stimulated A7r5 cells was dependent on angiotensin type I receptor (AT1R). Our study provided the first evidence that egg ovotransferrin-derived antihypertensive peptide IRW inhibited migration of VSMCs.

AB - Excessive proliferation, inflammation, oxidative stress, and migration induced by angiotensin II (Ang II), occurring in vascular smooth muscle cells (VSMCs) during vascular remodelling, are major pathogenesis of hypertension. Antihypertensive peptides derived from food proteins are promising alternatives in preventing/treating hypertension and associated complications. In addition to reducing high blood pressure in spontaneously hypertensive rats, egg white ovotransferrin-derived antihypertensive IRW (Ile-Arg-Trp) was shown to exert antiproliferative, antioxidant, and anti-inflammatory effects in A7r5 cells (a vascular smooth muscle cell line) against Ang II stimulation, further indicating its potential in retarding vascular remodelling. Since its regulatory role in migration of VSMC is unclear, the objective of this study was to evaluate the antimigrant activity of IRW in Ang II-stimulated A7r5 cells. It was found that IRW could downregulate matrix metallopeptidase 9 (MMP9) expression and inhibit migration of Ang II-stimulated A7r5 cells, which was associated with inactivation of p38/MAPK signaling. More importantly, the antimigrant activity of IRW in Ang II-stimulated A7r5 cells was dependent on angiotensin type I receptor (AT1R). Our study provided the first evidence that egg ovotransferrin-derived antihypertensive peptide IRW inhibited migration of VSMCs.

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KW - ovotransferrin

KW - vascular smooth muscle cell

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Egg White-Derived Antihypertensive Peptide IRW (Ile-Arg-Trp) Inhibits Angiotensin II-Stimulated Migration of Vascular Smooth Muscle Cells via Angiotensin Type i Receptor

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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