EHD1 - An EH-domain-containing protein with a specific expression pattern

Liat Mintz, Emilia Galperin, Metsada Pasmanik-Chor, Sandra Tulzinsky, Yael Bromberg, Christine A. Kozak, Alexandra Joyner, Amos Fein, Mia Horowitz

Research output: Contribution to journalArticlepeer-review

82 Scopus citations

Abstract

A cDNA that is a member of the eps15 homology (EH)-domain-containing family and is expressed differentially in testis was isolated from mouse and human. The corresponding genes map to the centromeric region of mouse chromosome 19 and to the region of conserved synteny on human chromosome 11q13. Northern analysis revealed two RNA species in mouse. In addition to the high levels in testis, expression was noted in kidney, heart, intestine, and brain. In human, three RNA species were evident. The smaller one was predominant in testis, while the largest species was evident in other tissues as well. The predicted protein sequence has an EH domain at its C-terminus, including an EF, a Ca2+ binding motif, and a central coiled-coil structure, as well as a nucleotide binding consensus site at its N-terminus. As such, it is a member of the EH-domain-containing protein family and was designated EHD1 (EH domain-containing 1). In cells in tissue culture, we localized EHD1 as a green fluorescent protein fusion protein, in transferrin-containing, endocytic vesicles. Immunostaining of different adult mouse organs revealed major expression of EHD1 in germ cells in meiosis, in the testes, in adipocytes, and in specific retinal layers. Results of in situ hybridization to whole embryos and immunohistochemical analyses indicated that EHD1 expression was already noted at day 9.5 in the limb buds and pharyngeal arches and at day 10.5 in sclerotomes, at various elements of the branchial apparatus (mandible and hyoid), and in the occipital region. At day 15.5 EHD1 expression peaked in cartilage, preceding hypertrophy and ossification, and at day 17.5 there was no expression in the bones. The EHD1 gene is highly conserved between nematode, Drosophila, mouse, and human. Its predicted protein structure and cellular localization point to the possibility that EHD1 participates in ligand-induced endocytosis.

Original languageEnglish
Pages (from-to)66-76
Number of pages11
JournalGenomics
Volume59
Issue number1
DOIs
StatePublished - Jul 1 1999

Bibliographical note

Funding Information:
We are grateful to Ms. Hassida Orenstein for preparation of mouse sections, to Dr. Nechama Smorodinsky and Dr. Naam Kariv for advice and help in the process of antibody preparation, and to Dr. Leonid Mitelman for help with the confocal microscopy. This work was partially funded by Grant 4231 from the Chief Scientist’s Office of the Ministry of Health, Israel (to M.H.).

ASJC Scopus subject areas

  • Genetics

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