Abstract
Background/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers.
Original language | English |
---|---|
Article number | 105028 |
Journal | Archives of Oral Biology |
Volume | 122 |
DOIs | |
State | Published - Feb 2021 |
Bibliographical note
Funding Information:The authors appreciate the financial support provided by the Research Funding Agency from São Paulo State (FAPESP), Brazil grants #2016/16102-5 and 2016/07528-9 ; CNPq - National Council for Scientific and Technological Development – Brazil for supporting Dr. Mauro P. Santamaria ( Grant #301102/2016-3 ). This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brazil (CAPES) – Finance Code 001 for supporting Dr. Camila Lopes Ferreira with a regular doctorate scholarship and an internship abroad scholarship (CAPES-PDSE).
Funding Information:
The authors appreciate the financial support provided by the Research Funding Agency from S?o Paulo State (FAPESP), Brazil grants #2016/16102-5 and 2016/07528-9; CNPq - National Council for Scientific and Technological Development ? Brazil for supporting Dr. Mauro P. Santamaria (Grant #301102/2016-3). This study was financed in part by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior ? Brazil (CAPES) ? Finance Code 001 for supporting Dr. Camila Lopes Ferreira with a regular doctorate scholarship and an internship abroad scholarship (CAPES-PDSE).
Publisher Copyright:
© 2020 Elsevier Ltd
Keywords
- Electrical stimulation
- Gingival recession
- Hard palate
- Oral mucosa
- Re-epithelialization
- Wound healing
ASJC Scopus subject areas
- Otorhinolaryngology
- Dentistry (all)
- Cell Biology