Electrical stimulation enhances early palatal wound healing in mice

Camila Lopes Ferreira; Maria Aparecida Neves Jardini; Camilla Magnoni Moretto Nunes; Daniella Vicensotto Bernardo; Renato Corrêa Viana Casarin; Estevão dos Santos Gedraite; Márcio Antônio Mathias; Fei Liu; Gustavo Mendonça; Daniela Baccelli Silveira Mendonça; Mauro Pedrine Santamaria

doi:10.1016/j.archoralbio.2020.105028

Electrical stimulation enhances early palatal wound healing in mice

Camila Lopes Ferreira, Maria Aparecida Neves Jardini, Camilla Magnoni Moretto Nunes, Daniella Vicensotto Bernardo, Renato Corrêa Viana Casarin, Estevão dos Santos Gedraite, Márcio Antônio Mathias, Fei Liu, Gustavo Mendonça, Daniela Baccelli Silveira Mendonça, Mauro Pedrine Santamaria

Oral Health Practice

Research output: Contribution to journal › Article › peer-review

Abstract

Background/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers.

Original language	English
Article number	105028
Journal	Archives of Oral Biology
Volume	122
DOIs	https://doi.org/10.1016/j.archoralbio.2020.105028
State	Published - Feb 2021

Bibliographical note

Funding Information:
The authors appreciate the financial support provided by the Research Funding Agency from São Paulo State (FAPESP), Brazil grants #2016/16102-5 and 2016/07528-9 ; CNPq - National Council for Scientific and Technological Development – Brazil for supporting Dr. Mauro P. Santamaria ( Grant #301102/2016-3 ). This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brazil (CAPES) – Finance Code 001 for supporting Dr. Camila Lopes Ferreira with a regular doctorate scholarship and an internship abroad scholarship (CAPES-PDSE).

Funding Information:
The authors appreciate the financial support provided by the Research Funding Agency from S?o Paulo State (FAPESP), Brazil grants #2016/16102-5 and 2016/07528-9; CNPq - National Council for Scientific and Technological Development ? Brazil for supporting Dr. Mauro P. Santamaria (Grant #301102/2016-3). This study was financed in part by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior ? Brazil (CAPES) ? Finance Code 001 for supporting Dr. Camila Lopes Ferreira with a regular doctorate scholarship and an internship abroad scholarship (CAPES-PDSE).

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

Electrical stimulation
Gingival recession
Hard palate
Oral mucosa
Re-epithelialization
Wound healing

ASJC Scopus subject areas

Otorhinolaryngology
Dentistry (all)
Cell Biology

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10.1016/j.archoralbio.2020.105028

Cite this

Ferreira, C. L., Neves Jardini, M. A., Moretto Nunes, C. M., Bernardo, D. V., Viana Casarin, R. C., dos Santos Gedraite, E., Mathias, M. A., Liu, F., Mendonça, G., Silveira Mendonça, D. B., & Santamaria, M. P. (2021). Electrical stimulation enhances early palatal wound healing in mice. Archives of Oral Biology, 122, [105028]. https://doi.org/10.1016/j.archoralbio.2020.105028

@article{117a2930c5d94525bb1a9a8329fb9a7b,

title = "Electrical stimulation enhances early palatal wound healing in mice",

abstract = "Background/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers.",

keywords = "Electrical stimulation, Gingival recession, Hard palate, Oral mucosa, Re-epithelialization, Wound healing",

author = "Ferreira, {Camila Lopes} and {Neves Jardini}, {Maria Aparecida} and {Moretto Nunes}, {Camilla Magnoni} and Bernardo, {Daniella Vicensotto} and {Viana Casarin}, {Renato Corr{\^e}a} and {dos Santos Gedraite}, Estev{\~a}o and Mathias, {M{\'a}rcio Ant{\^o}nio} and Fei Liu and Gustavo Mendon{\c c}a and {Silveira Mendon{\c c}a}, {Daniela Baccelli} and Santamaria, {Mauro Pedrine}",

note = "Funding Information: The authors appreciate the financial support provided by the Research Funding Agency from S{\~a}o Paulo State (FAPESP), Brazil grants #2016/16102-5 and 2016/07528-9 ; CNPq - National Council for Scientific and Technological Development – Brazil for supporting Dr. Mauro P. Santamaria ( Grant #301102/2016-3 ). This study was financed in part by the Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior – Brazil (CAPES) – Finance Code 001 for supporting Dr. Camila Lopes Ferreira with a regular doctorate scholarship and an internship abroad scholarship (CAPES-PDSE). Funding Information: The authors appreciate the financial support provided by the Research Funding Agency from S?o Paulo State (FAPESP), Brazil grants #2016/16102-5 and 2016/07528-9; CNPq - National Council for Scientific and Technological Development ? Brazil for supporting Dr. Mauro P. Santamaria (Grant #301102/2016-3). This study was financed in part by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior ? Brazil (CAPES) ? Finance Code 001 for supporting Dr. Camila Lopes Ferreira with a regular doctorate scholarship and an internship abroad scholarship (CAPES-PDSE). Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2021",

month = feb,

doi = "10.1016/j.archoralbio.2020.105028",

language = "English",

volume = "122",

}

TY - JOUR

T1 - Electrical stimulation enhances early palatal wound healing in mice

AU - Ferreira, Camila Lopes

AU - Neves Jardini, Maria Aparecida

AU - Moretto Nunes, Camilla Magnoni

AU - Bernardo, Daniella Vicensotto

AU - Viana Casarin, Renato Corrêa

AU - dos Santos Gedraite, Estevão

AU - Mathias, Márcio Antônio

AU - Liu, Fei

AU - Mendonça, Gustavo

AU - Silveira Mendonça, Daniela Baccelli

AU - Santamaria, Mauro Pedrine

N1 - Funding Information: The authors appreciate the financial support provided by the Research Funding Agency from São Paulo State (FAPESP), Brazil grants #2016/16102-5 and 2016/07528-9 ; CNPq - National Council for Scientific and Technological Development – Brazil for supporting Dr. Mauro P. Santamaria ( Grant #301102/2016-3 ). This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brazil (CAPES) – Finance Code 001 for supporting Dr. Camila Lopes Ferreira with a regular doctorate scholarship and an internship abroad scholarship (CAPES-PDSE). Funding Information: The authors appreciate the financial support provided by the Research Funding Agency from S?o Paulo State (FAPESP), Brazil grants #2016/16102-5 and 2016/07528-9; CNPq - National Council for Scientific and Technological Development ? Brazil for supporting Dr. Mauro P. Santamaria (Grant #301102/2016-3). This study was financed in part by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior ? Brazil (CAPES) ? Finance Code 001 for supporting Dr. Camila Lopes Ferreira with a regular doctorate scholarship and an internship abroad scholarship (CAPES-PDSE). Publisher Copyright: © 2020 Elsevier Ltd

PY - 2021/2

Y1 - 2021/2

N2 - Background/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers.

AB - Background/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers.

KW - Electrical stimulation

KW - Gingival recession

KW - Hard palate

KW - Oral mucosa

KW - Re-epithelialization

KW - Wound healing

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ER -

Electrical stimulation enhances early palatal wound healing in mice

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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