Electrical stimulation enhances early palatal wound healing in mice

Camila Lopes Ferreira, Maria Aparecida Neves Jardini, Camilla Magnoni Moretto Nunes, Daniella Vicensotto Bernardo, Renato Corrêa Viana Casarin, Estevão dos Santos Gedraite, Márcio Antônio Mathias, Fei Liu, Gustavo Mendonça, Daniela Baccelli Silveira Mendonça, Mauro Pedrine Santamaria

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Background/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers.

Original languageEnglish
Article number105028
JournalArchives of Oral Biology
StatePublished - Feb 2021

Bibliographical note

Publisher Copyright:
© 2020 Elsevier Ltd


  • Electrical stimulation
  • Gingival recession
  • Hard palate
  • Oral mucosa
  • Re-epithelialization
  • Wound healing

ASJC Scopus subject areas

  • Otorhinolaryngology
  • General Dentistry
  • Cell Biology


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