Electrical stimulation enhances tissue reorganization during orthodontic tooth movement in rats

Gisele Sampaio Spadari; Ewerton Zaniboni; Silvia Amelia Scudeler Vedovello; Mauro Pedrine Santamaria; Maria Esméria Corezola do Amaral; Gláucia Maria Tech dos Santos; Marcelo Augusto Marretto Esquisatto; Fernanda Aparecida Sampaio Mendonca; Milton Santamaria-Jr

doi:10.1007/s00784-016-1759-6

Electrical stimulation enhances tissue reorganization during orthodontic tooth movement in rats

Gisele Sampaio Spadari, Ewerton Zaniboni, Silvia Amelia Scudeler Vedovello, Mauro Pedrine Santamaria, Maria Esméria Corezola do Amaral, Gláucia Maria Tech dos Santos, Marcelo Augusto Marretto Esquisatto, Fernanda Aparecida Sampaio Mendonca, Milton Santamaria-Jr

Oral Health Practice

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Objective: This study evaluated the effects of a low-intensity electric current on tissue reorganization during experimental orthodontic tooth movement. Materials and methods: Thirty-two animals were divided into two groups evaluated on days 3 and 7: OTM—orthodontic tooth movement and OTM + MC—orthodontic tooth movement and microcurrent application (10 μA/5 min). The samples were processed for histological, morphometric, and Western blotting analysis. Results: Analysis of the periodontal ligament (PL) showed a significantly smaller number of granulocytes in the OTM + MC group on day 7.The number of fibroblasts was significantly higher in the OTM + MC group on days 3 and 7. The area of birefringent collagen fibers was more organized in the OTM + MC group on days 3 and 7. The number of blood vessels was significantly higher in the OTM + MC group on day 7. Microcurrent application significantly increased the number of osteoclasts in the compression region of the PL. In the OTM + MC group on day 7 of tooth movement, the expression of TGF-β1 and VEGF was significantly reduced whereas the expression of bFGF was increased in PL. Conclusions: Electrical stimulation enhances tissue responses, reducing the number of granulocytes and increasing the number of fibroblasts, blood vessels, and osteoclasts and modulates the expression of TGF-β1, VEFG, and bFGF. Clinical relevance: This technique is used in many areas of medicine, but poorly explored in dentistry and orthodontics. This treatment is cheap and non-invasive and can be applied by own orthodontist, and it can improve the treatment with a faster and safe tooth movement, without pain.

Original language	English
Pages (from-to)	111-120
Number of pages	10
Journal	Clinical Oral Investigations
Volume	21
Issue number	1
DOIs	https://doi.org/10.1007/s00784-016-1759-6
State	Published - Jan 1 2017

Bibliographical note

Funding Information:
This study was funded by the National Council for Scientific and Technological Development - CAPES/PNPD (process no. 23038.008192/2013-01) and Heminio Ometto University Center.

Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.

Keywords

Low-intensity electric current
Microcurrent application
Orthodontic tooth movement

ASJC Scopus subject areas

Dentistry (all)

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10.1007/s00784-016-1759-6

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title = "Electrical stimulation enhances tissue reorganization during orthodontic tooth movement in rats",

abstract = "Objective: This study evaluated the effects of a low-intensity electric current on tissue reorganization during experimental orthodontic tooth movement. Materials and methods: Thirty-two animals were divided into two groups evaluated on days 3 and 7: OTM—orthodontic tooth movement and OTM + MC—orthodontic tooth movement and microcurrent application (10 μA/5 min). The samples were processed for histological, morphometric, and Western blotting analysis. Results: Analysis of the periodontal ligament (PL) showed a significantly smaller number of granulocytes in the OTM + MC group on day 7.The number of fibroblasts was significantly higher in the OTM + MC group on days 3 and 7. The area of birefringent collagen fibers was more organized in the OTM + MC group on days 3 and 7. The number of blood vessels was significantly higher in the OTM + MC group on day 7. Microcurrent application significantly increased the number of osteoclasts in the compression region of the PL. In the OTM + MC group on day 7 of tooth movement, the expression of TGF-β1 and VEGF was significantly reduced whereas the expression of bFGF was increased in PL. Conclusions: Electrical stimulation enhances tissue responses, reducing the number of granulocytes and increasing the number of fibroblasts, blood vessels, and osteoclasts and modulates the expression of TGF-β1, VEFG, and bFGF. Clinical relevance: This technique is used in many areas of medicine, but poorly explored in dentistry and orthodontics. This treatment is cheap and non-invasive and can be applied by own orthodontist, and it can improve the treatment with a faster and safe tooth movement, without pain.",

keywords = "Low-intensity electric current, Microcurrent application, Orthodontic tooth movement",

author = "Spadari, {Gisele Sampaio} and Ewerton Zaniboni and Vedovello, {Silvia Amelia Scudeler} and Santamaria, {Mauro Pedrine} and {do Amaral}, {Maria Esm{\'e}ria Corezola} and {dos Santos}, {Gl{\'a}ucia Maria Tech} and Esquisatto, {Marcelo Augusto Marretto} and Mendonca, {Fernanda Aparecida Sampaio} and Milton Santamaria-Jr",

note = "Funding Information: This study was funded by the National Council for Scientific and Technological Development - CAPES/PNPD (process no. 23038.008192/2013-01) and Heminio Ometto University Center. Publisher Copyright: {\textcopyright} 2016, Springer-Verlag Berlin Heidelberg.",

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T1 - Electrical stimulation enhances tissue reorganization during orthodontic tooth movement in rats

AU - Spadari, Gisele Sampaio

AU - Zaniboni, Ewerton

AU - Vedovello, Silvia Amelia Scudeler

AU - Santamaria, Mauro Pedrine

AU - do Amaral, Maria Esméria Corezola

AU - dos Santos, Gláucia Maria Tech

AU - Esquisatto, Marcelo Augusto Marretto

AU - Mendonca, Fernanda Aparecida Sampaio

AU - Santamaria-Jr, Milton

N1 - Funding Information: This study was funded by the National Council for Scientific and Technological Development - CAPES/PNPD (process no. 23038.008192/2013-01) and Heminio Ometto University Center. Publisher Copyright: © 2016, Springer-Verlag Berlin Heidelberg.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Objective: This study evaluated the effects of a low-intensity electric current on tissue reorganization during experimental orthodontic tooth movement. Materials and methods: Thirty-two animals were divided into two groups evaluated on days 3 and 7: OTM—orthodontic tooth movement and OTM + MC—orthodontic tooth movement and microcurrent application (10 μA/5 min). The samples were processed for histological, morphometric, and Western blotting analysis. Results: Analysis of the periodontal ligament (PL) showed a significantly smaller number of granulocytes in the OTM + MC group on day 7.The number of fibroblasts was significantly higher in the OTM + MC group on days 3 and 7. The area of birefringent collagen fibers was more organized in the OTM + MC group on days 3 and 7. The number of blood vessels was significantly higher in the OTM + MC group on day 7. Microcurrent application significantly increased the number of osteoclasts in the compression region of the PL. In the OTM + MC group on day 7 of tooth movement, the expression of TGF-β1 and VEGF was significantly reduced whereas the expression of bFGF was increased in PL. Conclusions: Electrical stimulation enhances tissue responses, reducing the number of granulocytes and increasing the number of fibroblasts, blood vessels, and osteoclasts and modulates the expression of TGF-β1, VEFG, and bFGF. Clinical relevance: This technique is used in many areas of medicine, but poorly explored in dentistry and orthodontics. This treatment is cheap and non-invasive and can be applied by own orthodontist, and it can improve the treatment with a faster and safe tooth movement, without pain.

AB - Objective: This study evaluated the effects of a low-intensity electric current on tissue reorganization during experimental orthodontic tooth movement. Materials and methods: Thirty-two animals were divided into two groups evaluated on days 3 and 7: OTM—orthodontic tooth movement and OTM + MC—orthodontic tooth movement and microcurrent application (10 μA/5 min). The samples were processed for histological, morphometric, and Western blotting analysis. Results: Analysis of the periodontal ligament (PL) showed a significantly smaller number of granulocytes in the OTM + MC group on day 7.The number of fibroblasts was significantly higher in the OTM + MC group on days 3 and 7. The area of birefringent collagen fibers was more organized in the OTM + MC group on days 3 and 7. The number of blood vessels was significantly higher in the OTM + MC group on day 7. Microcurrent application significantly increased the number of osteoclasts in the compression region of the PL. In the OTM + MC group on day 7 of tooth movement, the expression of TGF-β1 and VEGF was significantly reduced whereas the expression of bFGF was increased in PL. Conclusions: Electrical stimulation enhances tissue responses, reducing the number of granulocytes and increasing the number of fibroblasts, blood vessels, and osteoclasts and modulates the expression of TGF-β1, VEFG, and bFGF. Clinical relevance: This technique is used in many areas of medicine, but poorly explored in dentistry and orthodontics. This treatment is cheap and non-invasive and can be applied by own orthodontist, and it can improve the treatment with a faster and safe tooth movement, without pain.

KW - Low-intensity electric current

KW - Microcurrent application

KW - Orthodontic tooth movement

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Electrical stimulation enhances tissue reorganization during orthodontic tooth movement in rats

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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