Electrochemical fabrication and evaluation of highly sensitive nanorod-modified electrodes for a biotin/avidin system

Seung Jun Lee, Venkataramani Anandan, Guigen Zhang

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Bioaffinity sensors need to be rapid, specific, and highly sensitive. To realize these features, electrodes that can elicit high electrochemical performance are necessary. In this study, we developed nanorod array electrode and performed cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments to study the interfacial properties of the nanorod array electrode with Fe(CN)63-/4- as the redox molecules. Results showed that both the CV and EIS measurements captured very well the resistive and capacitive changes due to the adsorption of functionalizing molecules and the coupling between avidin and biotin. The EIS measurements were more sensitive in discriminating small changes caused by the surface adsorption of various molecules. The use of avidin-functionalized gold nanorod modified electrodes had led to much increased detection sensitivity along with a detection-limit as low as 1 ng/mL of biotin.

Original languageEnglish
Pages (from-to)1117-1124
Number of pages8
JournalBiosensors and Bioelectronics
Volume23
Issue number7
DOIs
StatePublished - Feb 28 2008

Bibliographical note

Funding Information:
This work was supported by the National Science Foundation (ECS 0304340) and the Korea Research Foundation (MOEHRD: KRF 2006-214-D00029).

Keywords

  • Avidin/biotin
  • Bioaffinity
  • Cyclic voltammetry
  • Electrochemical impedance spectroscopy
  • Nanorod electrodes

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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