TY - JOUR
T1 - Electron spin resonance investigation of laser and heated metal‐tip–induced free radical formation in various tissues
AU - Jain, Sandeep
AU - Shi, Xianglin
AU - Billie, Mike
AU - Jain, Abnash C.
AU - Dalal, Nar S.
PY - 1989
Y1 - 1989
N2 - The short‐lived free radical formation accompanying laser irradiation and laser‐heated metal tip contact was examined using electron spin resonance (ESR) spin‐trap methodology. Various tissues (canine myocardium, human aorta, liver, and spleen) were irradiated by argon‐ion (continuous wave [CW] and pulse) and YAG (CW) lasers employing both naked fiber and a laser‐heated metal tip. All showed the formation of primarily carbon‐centered, not oxygenated, free radicals, together with some minor unidentified species. This implies that the backbones of amino acids, lipids, or other biological building blocks are cleaved during the irradiation or thermal treatment. Different tissues produce similar radicals but with different amounts when irradiated by argon‐ion, YAG, and laser‐heated metal tip sources. The amount of the free radicals formed depends on the laser power (within 5–15 W). Compared to the naked fiber, the laser‐heated metal tip shows the generation of at least twice the amount of free radicals. The possible relationship of the free radical formation to tissue injury is briefly discussed.
AB - The short‐lived free radical formation accompanying laser irradiation and laser‐heated metal tip contact was examined using electron spin resonance (ESR) spin‐trap methodology. Various tissues (canine myocardium, human aorta, liver, and spleen) were irradiated by argon‐ion (continuous wave [CW] and pulse) and YAG (CW) lasers employing both naked fiber and a laser‐heated metal tip. All showed the formation of primarily carbon‐centered, not oxygenated, free radicals, together with some minor unidentified species. This implies that the backbones of amino acids, lipids, or other biological building blocks are cleaved during the irradiation or thermal treatment. Different tissues produce similar radicals but with different amounts when irradiated by argon‐ion, YAG, and laser‐heated metal tip sources. The amount of the free radicals formed depends on the laser power (within 5–15 W). Compared to the naked fiber, the laser‐heated metal tip shows the generation of at least twice the amount of free radicals. The possible relationship of the free radical formation to tissue injury is briefly discussed.
KW - ESR
KW - laser‐heated metal‐tip
KW - spin trapping
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U2 - 10.1002/lsm.1900090612
DO - 10.1002/lsm.1900090612
M3 - Article
C2 - 2557503
AN - SCOPUS:0024818553
SN - 0196-8092
VL - 9
SP - 616
EP - 622
JO - Lasers in Surgery and Medicine
JF - Lasers in Surgery and Medicine
IS - 6
ER -