Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

Claudio W. Pikielny; Brian C. Rymond; Michael Rosbash

doi:10.1038/324341a0

Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

Claudio W. Pikielny, Brian C. Rymond, Michael Rosbash

Biology

Research output: Contribution to journal › Article › peer-review

184 Scopus citations

Abstract

Three splicing complexes formed with a yeast pre-messenger RNA during in vitro splicing can be resolved by non-denaturing gel electrophoresis after incubation in the presence of non-specific competitor RNA. The time course of the appearance of these complexes and their composition suggest that they represent an ordered pathway of splicing complex assembly.

Original language	English
Pages (from-to)	341-345
Number of pages	5
Journal	Nature
Volume	324
Issue number	6095
DOIs	https://doi.org/10.1038/324341a0
State	Published - 1986

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Funders	Funder number
National Institute of General Medical Sciences	R01GM023549

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title = "Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes",

abstract = "Three splicing complexes formed with a yeast pre-messenger RNA during in vitro splicing can be resolved by non-denaturing gel electrophoresis after incubation in the presence of non-specific competitor RNA. The time course of the appearance of these complexes and their composition suggest that they represent an ordered pathway of splicing complex assembly.",

author = "Pikielny, {Claudio W.} and Rymond, {Brian C.} and Michael Rosbash",

year = "1986",

doi = "10.1038/324341a0",

language = "English",

volume = "324",

pages = "341--345",

number = "6095",

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T1 - Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

AU - Pikielny, Claudio W.

AU - Rymond, Brian C.

AU - Rosbash, Michael

PY - 1986

Y1 - 1986

N2 - Three splicing complexes formed with a yeast pre-messenger RNA during in vitro splicing can be resolved by non-denaturing gel electrophoresis after incubation in the presence of non-specific competitor RNA. The time course of the appearance of these complexes and their composition suggest that they represent an ordered pathway of splicing complex assembly.

AB - Three splicing complexes formed with a yeast pre-messenger RNA during in vitro splicing can be resolved by non-denaturing gel electrophoresis after incubation in the presence of non-specific competitor RNA. The time course of the appearance of these complexes and their composition suggest that they represent an ordered pathway of splicing complex assembly.

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U2 - 10.1038/324341a0

DO - 10.1038/324341a0

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AN - SCOPUS:0022997930

SN - 0028-0836

VL - 324

SP - 341

EP - 345

JO - Nature

JF - Nature

IS - 6095

ER -

Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

Abstract

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