Electrophysiological characteristics of identified kidney-related neurons in adult rat spinal cord slices

Andrei V. Derbenev; Hanad Duale; Alexander G. Rabchevsky; Bret N. Smith

doi:10.1016/j.neulet.2010.03.036

Electrophysiological characteristics of identified kidney-related neurons in adult rat spinal cord slices

Andrei V. Derbenev
, Hanad Duale
, Alexander G. Rabchevsky
, Bret N. Smith

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Whole-cell patch-clamp recordings were made from kidney-related neurons in the intermediolateral cell column (IML) in horizontal slices of thoracolumbar spinal cord from adult rats. Kidney-related neurons were identified in vitro subsequent to inoculation of the kidney with a fluorescent, retrograde, transynaptic pseudorabies viral label (i.e., PRV-152). Kidney-related neurons detected in the IML expressed choline acetyltransferase, characteristic of spinal preganglionic motor neurons. Their mean resting potential was -51±4mV and input resistance was 448±39MΩ. Both spontaneous inhibitory and excitatory post-synaptic currents (i.e., sIPSCs and sEPSCs) were observed in all neurons. The mean frequency for sEPSCs (3.1±1Hz) was approximately 2.5 times that for sIPSCs (1.4±0.3Hz). Application of the glycine and GABA_A receptor-linked Cl^- channel blocker, picrotoxin (100μM) blocked sIPSCs, while the ionotropic glutamate receptor antagonist, kynurenic acid (1mM) blocked all sEPSCs, indicating they were mediated by GABA/glycine and glutamate receptors, respectively. Thus, using PRV-152 labeling allowed whole-cell patch-clamp recording of neurons in the adult spinal cord, which were kidney-related. Excitatory glutamatergic input dominated synaptic responses in these cells, the membrane characteristics of which resembled those of immature IML neurons. Combined PRV-152 pre-labeling and whole-cell patch-clamp recordings may allow more effective analysis of synaptic plasticity seen in adult models of injury or chronic disease.

Original language	English
Pages (from-to)	168-172
Number of pages	5
Journal	Neuroscience Letters
Volume	474
Issue number	3
DOIs	https://doi.org/10.1016/j.neulet.2010.03.036
State	Published - May 2010

Bibliographical note

Funding Information:
We thank Dr. Lynn Enquist (Princeton University) for the PRV-152. Funded by PVA grant # 2561 (H. Duale), NIHLBI , 1R21HL091293 (A.V. Derbenev), NINDS , R01 NS049901 (A.G. Rabchevsky), NIDDK , R01 DK056132 (B.N. Smith), and NINDS , P30 NS051220 .

Funding

We thank Dr. Lynn Enquist (Princeton University) for the PRV-152. Funded by PVA grant # 2561 (H. Duale), NIHLBI , 1R21HL091293 (A.V. Derbenev), NINDS , R01 NS049901 (A.G. Rabchevsky), NIDDK , R01 DK056132 (B.N. Smith), and NINDS , P30 NS051220 .

Funders	Funder number
NIHLBI	1R21HL091293
National Institute of Diabetes and Digestive and Kidney Diseases	R01 DK056132
National Institute of Neurological Disorders and Stroke	R01 NS049901, P30NS051220
Paralyzed Veterans of America	2561

Keywords

Intermediolateral cell column
Preganglionic neuron
Pseudorabies virus
Sympathetic
Whole-cell patch-clamp

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1016/j.neulet.2010.03.036

Cite this

@article{2f8e7c550fd749eeaceef1d48d1a1ed7,

title = "Electrophysiological characteristics of identified kidney-related neurons in adult rat spinal cord slices",

abstract = "Whole-cell patch-clamp recordings were made from kidney-related neurons in the intermediolateral cell column (IML) in horizontal slices of thoracolumbar spinal cord from adult rats. Kidney-related neurons were identified in vitro subsequent to inoculation of the kidney with a fluorescent, retrograde, transynaptic pseudorabies viral label (i.e., PRV-152). Kidney-related neurons detected in the IML expressed choline acetyltransferase, characteristic of spinal preganglionic motor neurons. Their mean resting potential was -51±4mV and input resistance was 448±39MΩ. Both spontaneous inhibitory and excitatory post-synaptic currents (i.e., sIPSCs and sEPSCs) were observed in all neurons. The mean frequency for sEPSCs (3.1±1Hz) was approximately 2.5 times that for sIPSCs (1.4±0.3Hz). Application of the glycine and GABAA receptor-linked Cl- channel blocker, picrotoxin (100μM) blocked sIPSCs, while the ionotropic glutamate receptor antagonist, kynurenic acid (1mM) blocked all sEPSCs, indicating they were mediated by GABA/glycine and glutamate receptors, respectively. Thus, using PRV-152 labeling allowed whole-cell patch-clamp recording of neurons in the adult spinal cord, which were kidney-related. Excitatory glutamatergic input dominated synaptic responses in these cells, the membrane characteristics of which resembled those of immature IML neurons. Combined PRV-152 pre-labeling and whole-cell patch-clamp recordings may allow more effective analysis of synaptic plasticity seen in adult models of injury or chronic disease.",

keywords = "Intermediolateral cell column, Preganglionic neuron, Pseudorabies virus, Sympathetic, Whole-cell patch-clamp",

author = "Derbenev, \{Andrei V.\} and Hanad Duale and Rabchevsky, \{Alexander G.\} and Smith, \{Bret N.\}",

note = "Funding Information: We thank Dr. Lynn Enquist (Princeton University) for the PRV-152. Funded by PVA grant \# 2561 (H. Duale), NIHLBI , 1R21HL091293 (A.V. Derbenev), NINDS , R01 NS049901 (A.G. Rabchevsky), NIDDK , R01 DK056132 (B.N. Smith), and NINDS , P30 NS051220 .",

year = "2010",

month = may,

doi = "10.1016/j.neulet.2010.03.036",

language = "English",

volume = "474",

pages = "168--172",

number = "3",

}

TY - JOUR

T1 - Electrophysiological characteristics of identified kidney-related neurons in adult rat spinal cord slices

AU - Derbenev, Andrei V.

AU - Duale, Hanad

AU - Rabchevsky, Alexander G.

AU - Smith, Bret N.

N1 - Funding Information: We thank Dr. Lynn Enquist (Princeton University) for the PRV-152. Funded by PVA grant # 2561 (H. Duale), NIHLBI , 1R21HL091293 (A.V. Derbenev), NINDS , R01 NS049901 (A.G. Rabchevsky), NIDDK , R01 DK056132 (B.N. Smith), and NINDS , P30 NS051220 .

PY - 2010/5

Y1 - 2010/5

N2 - Whole-cell patch-clamp recordings were made from kidney-related neurons in the intermediolateral cell column (IML) in horizontal slices of thoracolumbar spinal cord from adult rats. Kidney-related neurons were identified in vitro subsequent to inoculation of the kidney with a fluorescent, retrograde, transynaptic pseudorabies viral label (i.e., PRV-152). Kidney-related neurons detected in the IML expressed choline acetyltransferase, characteristic of spinal preganglionic motor neurons. Their mean resting potential was -51±4mV and input resistance was 448±39MΩ. Both spontaneous inhibitory and excitatory post-synaptic currents (i.e., sIPSCs and sEPSCs) were observed in all neurons. The mean frequency for sEPSCs (3.1±1Hz) was approximately 2.5 times that for sIPSCs (1.4±0.3Hz). Application of the glycine and GABAA receptor-linked Cl- channel blocker, picrotoxin (100μM) blocked sIPSCs, while the ionotropic glutamate receptor antagonist, kynurenic acid (1mM) blocked all sEPSCs, indicating they were mediated by GABA/glycine and glutamate receptors, respectively. Thus, using PRV-152 labeling allowed whole-cell patch-clamp recording of neurons in the adult spinal cord, which were kidney-related. Excitatory glutamatergic input dominated synaptic responses in these cells, the membrane characteristics of which resembled those of immature IML neurons. Combined PRV-152 pre-labeling and whole-cell patch-clamp recordings may allow more effective analysis of synaptic plasticity seen in adult models of injury or chronic disease.

AB - Whole-cell patch-clamp recordings were made from kidney-related neurons in the intermediolateral cell column (IML) in horizontal slices of thoracolumbar spinal cord from adult rats. Kidney-related neurons were identified in vitro subsequent to inoculation of the kidney with a fluorescent, retrograde, transynaptic pseudorabies viral label (i.e., PRV-152). Kidney-related neurons detected in the IML expressed choline acetyltransferase, characteristic of spinal preganglionic motor neurons. Their mean resting potential was -51±4mV and input resistance was 448±39MΩ. Both spontaneous inhibitory and excitatory post-synaptic currents (i.e., sIPSCs and sEPSCs) were observed in all neurons. The mean frequency for sEPSCs (3.1±1Hz) was approximately 2.5 times that for sIPSCs (1.4±0.3Hz). Application of the glycine and GABAA receptor-linked Cl- channel blocker, picrotoxin (100μM) blocked sIPSCs, while the ionotropic glutamate receptor antagonist, kynurenic acid (1mM) blocked all sEPSCs, indicating they were mediated by GABA/glycine and glutamate receptors, respectively. Thus, using PRV-152 labeling allowed whole-cell patch-clamp recording of neurons in the adult spinal cord, which were kidney-related. Excitatory glutamatergic input dominated synaptic responses in these cells, the membrane characteristics of which resembled those of immature IML neurons. Combined PRV-152 pre-labeling and whole-cell patch-clamp recordings may allow more effective analysis of synaptic plasticity seen in adult models of injury or chronic disease.

KW - Intermediolateral cell column

KW - Preganglionic neuron

KW - Pseudorabies virus

KW - Sympathetic

KW - Whole-cell patch-clamp

UR - https://www.scopus.com/pages/publications/77951499156

UR - https://www.scopus.com/pages/publications/77951499156#tab=citedBy

U2 - 10.1016/j.neulet.2010.03.036

DO - 10.1016/j.neulet.2010.03.036

M3 - Article

C2 - 20303390

AN - SCOPUS:77951499156

SN - 0304-3940

VL - 474

SP - 168

EP - 172

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 3

ER -

Electrophysiological characteristics of identified kidney-related neurons in adult rat spinal cord slices

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this