Recent studies have indicated that ceramide generated in the liver is secreted into the bloodstream as component of very-low-density lipoproteins (VLDL) and low-density lipoproteins (LDL). This manuscript investigates the effect of host acute phase response to inflammation on lipoprotein ceramide levels. In humans, two different patterns of responses were found. One group of volunteers experienced transient increases in serum ceramide at 1.5h after LPS administration. Second group showed prolonged increases that reached up to 10-fold above the basal level and continued for up to 24h. Increases in ceramide were found only in VLDL and LDL particles. LPS administration induced similar increases in mice. These increases were accompanied by activation of secreted sphingomyelinase in serum and serine-palmitoyl transferase in liver. ASMase knockout mice retained LPS-induced increases in serum ceramide, thus suggesting that the elevation of VLDL and LDL ceramide content is attributed at least in part to activation of de novo synthesis of ceramide in the liver.
|Number of pages||9|
|Journal||Archives of Biochemistry and Biophysics|
|State||Published - Nov 15 2003|
Bibliographical noteFunding Information:
This work was supported partly by research award from the American Federation for Aging Research (to M.N.-K) and a Grant-in-Aid from the American Heart Association (Ohio Valley affiliate (to M.N.-K), as well as by the Department of Veterans Affairs Merit Award Program (to S.I.S.) and by M01RR02602 NIH Grant for the University of Kentucky General Clinical Research Center (to S.I.S.). We greatly appreciate the comments of the Cardiovascular Research Group.
ASJC Scopus subject areas
- Molecular Biology