Elevation of ceramide in serum lipoproteins during acute phase response in humans and mice: Role of serine-palmitoyl transferase

Sandy Lightle; Raina Tosheva; Amy Lee; Jennie Queen-Baker; Boris Boyanovsky; Steve Shedlofsky; Mariana Nikolova-Karakashian

doi:10.1016/j.abb.2003.08.031

Elevation of ceramide in serum lipoproteins during acute phase response in humans and mice: Role of serine-palmitoyl transferase

Sandy Lightle, Raina Tosheva, Amy Lee, Jennie Queen-Baker, Boris Boyanovsky, Steve Shedlofsky, Mariana Nikolova-Karakashian

Physiology

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Recent studies have indicated that ceramide generated in the liver is secreted into the bloodstream as component of very-low-density lipoproteins (VLDL) and low-density lipoproteins (LDL). This manuscript investigates the effect of host acute phase response to inflammation on lipoprotein ceramide levels. In humans, two different patterns of responses were found. One group of volunteers experienced transient increases in serum ceramide at 1.5h after LPS administration. Second group showed prolonged increases that reached up to 10-fold above the basal level and continued for up to 24h. Increases in ceramide were found only in VLDL and LDL particles. LPS administration induced similar increases in mice. These increases were accompanied by activation of secreted sphingomyelinase in serum and serine-palmitoyl transferase in liver. ASMase knockout mice retained LPS-induced increases in serum ceramide, thus suggesting that the elevation of VLDL and LDL ceramide content is attributed at least in part to activation of de novo synthesis of ceramide in the liver.

Original language	English
Pages (from-to)	120-128
Number of pages	9
Journal	Archives of Biochemistry and Biophysics
Volume	419
Issue number	2
DOIs	https://doi.org/10.1016/j.abb.2003.08.031
State	Published - Nov 15 2003

Bibliographical note

Funding Information:
This work was supported partly by research award from the American Federation for Aging Research (to M.N.-K) and a Grant-in-Aid from the American Heart Association (Ohio Valley affiliate (to M.N.-K), as well as by the Department of Veterans Affairs Merit Award Program (to S.I.S.) and by M01RR02602 NIH Grant for the University of Kentucky General Clinical Research Center (to S.I.S.). We greatly appreciate the comments of the Cardiovascular Research Group.

Keywords

Atherosclerosis
LPS
Liver
Sphingomyelinase
Sphingosine

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/j.abb.2003.08.031

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title = "Elevation of ceramide in serum lipoproteins during acute phase response in humans and mice: Role of serine-palmitoyl transferase",

abstract = "Recent studies have indicated that ceramide generated in the liver is secreted into the bloodstream as component of very-low-density lipoproteins (VLDL) and low-density lipoproteins (LDL). This manuscript investigates the effect of host acute phase response to inflammation on lipoprotein ceramide levels. In humans, two different patterns of responses were found. One group of volunteers experienced transient increases in serum ceramide at 1.5h after LPS administration. Second group showed prolonged increases that reached up to 10-fold above the basal level and continued for up to 24h. Increases in ceramide were found only in VLDL and LDL particles. LPS administration induced similar increases in mice. These increases were accompanied by activation of secreted sphingomyelinase in serum and serine-palmitoyl transferase in liver. ASMase knockout mice retained LPS-induced increases in serum ceramide, thus suggesting that the elevation of VLDL and LDL ceramide content is attributed at least in part to activation of de novo synthesis of ceramide in the liver.",

keywords = "Atherosclerosis, LPS, Liver, Sphingomyelinase, Sphingosine",

author = "Sandy Lightle and Raina Tosheva and Amy Lee and Jennie Queen-Baker and Boris Boyanovsky and Steve Shedlofsky and Mariana Nikolova-Karakashian",

note = "Funding Information: This work was supported partly by research award from the American Federation for Aging Research (to M.N.-K) and a Grant-in-Aid from the American Heart Association (Ohio Valley affiliate (to M.N.-K), as well as by the Department of Veterans Affairs Merit Award Program (to S.I.S.) and by M01RR02602 NIH Grant for the University of Kentucky General Clinical Research Center (to S.I.S.). We greatly appreciate the comments of the Cardiovascular Research Group.",

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language = "English",

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AU - Lightle, Sandy

AU - Tosheva, Raina

AU - Lee, Amy

AU - Queen-Baker, Jennie

AU - Boyanovsky, Boris

AU - Shedlofsky, Steve

AU - Nikolova-Karakashian, Mariana

N1 - Funding Information: This work was supported partly by research award from the American Federation for Aging Research (to M.N.-K) and a Grant-in-Aid from the American Heart Association (Ohio Valley affiliate (to M.N.-K), as well as by the Department of Veterans Affairs Merit Award Program (to S.I.S.) and by M01RR02602 NIH Grant for the University of Kentucky General Clinical Research Center (to S.I.S.). We greatly appreciate the comments of the Cardiovascular Research Group.

PY - 2003/11/15

Y1 - 2003/11/15

N2 - Recent studies have indicated that ceramide generated in the liver is secreted into the bloodstream as component of very-low-density lipoproteins (VLDL) and low-density lipoproteins (LDL). This manuscript investigates the effect of host acute phase response to inflammation on lipoprotein ceramide levels. In humans, two different patterns of responses were found. One group of volunteers experienced transient increases in serum ceramide at 1.5h after LPS administration. Second group showed prolonged increases that reached up to 10-fold above the basal level and continued for up to 24h. Increases in ceramide were found only in VLDL and LDL particles. LPS administration induced similar increases in mice. These increases were accompanied by activation of secreted sphingomyelinase in serum and serine-palmitoyl transferase in liver. ASMase knockout mice retained LPS-induced increases in serum ceramide, thus suggesting that the elevation of VLDL and LDL ceramide content is attributed at least in part to activation of de novo synthesis of ceramide in the liver.

AB - Recent studies have indicated that ceramide generated in the liver is secreted into the bloodstream as component of very-low-density lipoproteins (VLDL) and low-density lipoproteins (LDL). This manuscript investigates the effect of host acute phase response to inflammation on lipoprotein ceramide levels. In humans, two different patterns of responses were found. One group of volunteers experienced transient increases in serum ceramide at 1.5h after LPS administration. Second group showed prolonged increases that reached up to 10-fold above the basal level and continued for up to 24h. Increases in ceramide were found only in VLDL and LDL particles. LPS administration induced similar increases in mice. These increases were accompanied by activation of secreted sphingomyelinase in serum and serine-palmitoyl transferase in liver. ASMase knockout mice retained LPS-induced increases in serum ceramide, thus suggesting that the elevation of VLDL and LDL ceramide content is attributed at least in part to activation of de novo synthesis of ceramide in the liver.

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KW - Sphingosine

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Elevation of ceramide in serum lipoproteins during acute phase response in humans and mice: Role of serine-palmitoyl transferase

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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