TY - JOUR
T1 - Elevation of cytosolic calcium is sufficient to induce growth inhibition in a B cell lymphoma
AU - Muthukkumar, Subramanian
AU - Udhayakumar, Venkatachalam
AU - Bondada, Subbarao
PY - 1993/10
Y1 - 1993/10
N2 - Recently, we have described that anti‐IgM antibodies profoundly inhibited the growth of BKS‐2, an immature B cell lymphoma. In this report, we demonstrated that ionomycin alone at very low concentrations (20 nM) inhibited the growth of BKS‐2 cells completely. The levels of intracellular Ca2+ induced by the inhibitory concentrations of ionomycin were comparable to those in anti‐IgM‐treated cells. The growth inhibition caused by ionomycin was reversed by phorbol 12‐myristate 13‐acetate and lipopolysaccharide. In addition, the immunosuppressants, cyclosporin A and FK506 conferred significant protection from the negative signal induced by ionomycin. However, either cyclosporin A, FK506 or lipopolysaccharide was not found to have direct effect on ionomycin‐induced Ca2+ mobilization in BKS‐2 cells. Also, ionomycin augmented the anti‐IgM‐induced growth arrest in these cells. Furthermore, BKS‐2 cells that were exposed to anti‐IgM or ionomycin underwent apoptosis as characterized by DNA fragmentation. Thus, the characteristics of growth inhibition induced by ionomycin and anti‐IgM appeared to be similar in that phorbol 12‐myristate 13‐acetate, lipopolysaccharide, cyclosporin A and FK506 caused significant reversal from such negative signals and both ionomycin and anti‐IgM induced apoptosis in these cells. Altogether, these results showed that the elevation of intracellular Ca2+ alone was sufficient to inhibit the growth of some B lymphoma cells.
AB - Recently, we have described that anti‐IgM antibodies profoundly inhibited the growth of BKS‐2, an immature B cell lymphoma. In this report, we demonstrated that ionomycin alone at very low concentrations (20 nM) inhibited the growth of BKS‐2 cells completely. The levels of intracellular Ca2+ induced by the inhibitory concentrations of ionomycin were comparable to those in anti‐IgM‐treated cells. The growth inhibition caused by ionomycin was reversed by phorbol 12‐myristate 13‐acetate and lipopolysaccharide. In addition, the immunosuppressants, cyclosporin A and FK506 conferred significant protection from the negative signal induced by ionomycin. However, either cyclosporin A, FK506 or lipopolysaccharide was not found to have direct effect on ionomycin‐induced Ca2+ mobilization in BKS‐2 cells. Also, ionomycin augmented the anti‐IgM‐induced growth arrest in these cells. Furthermore, BKS‐2 cells that were exposed to anti‐IgM or ionomycin underwent apoptosis as characterized by DNA fragmentation. Thus, the characteristics of growth inhibition induced by ionomycin and anti‐IgM appeared to be similar in that phorbol 12‐myristate 13‐acetate, lipopolysaccharide, cyclosporin A and FK506 caused significant reversal from such negative signals and both ionomycin and anti‐IgM induced apoptosis in these cells. Altogether, these results showed that the elevation of intracellular Ca2+ alone was sufficient to inhibit the growth of some B lymphoma cells.
KW - Anti‐IgM
KW - B cell lymphoma
KW - Growth inhibition
KW - Intracellular Ca2
KW - Ionomycin
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U2 - 10.1002/eji.1830231007
DO - 10.1002/eji.1830231007
M3 - Article
C2 - 7691606
AN - SCOPUS:0027360451
SN - 0014-2980
VL - 23
SP - 2419
EP - 2426
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 10
ER -