Elucidation of the glycosylation sequence of mithramycin biosynthesis: Isolation of 3A-deolivosylpremithramycin B and its conversion to premithramycin B by glycosyltransferase MtmGII

Mohammad Nur-E-Alam, Carmen Méndez, José A. Salas, Jürgen Rohr

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The last step at last. The isolation and structure elucidation of the novel premithramycin-type compound 3A-deolivosylpremithramycin B (1) allowed cross-feeding experiments with this compound to two glycosyltransferase-minus mutants of the mithramycin producer S. argillaceus; this has solved the sequence of the last glycosylation steps of mithramycin biosynthesis.

Original languageEnglish
Pages (from-to)632-636
Number of pages5
JournalChemBioChem
Volume6
Issue number4
DOIs
StatePublished - Apr 2005

Keywords

  • Biosynthesis
  • Glycosylation
  • Glycosyltransferases
  • Mithramycin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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