Embryonic hyperglycemia perturbs the development of specific retinal cell types, including photoreceptors

Kayla F. Titialii-Torres; Ann C. Morris

doi:10.1242/jcs.259187

Embryonic hyperglycemia perturbs the development of specific retinal cell types, including photoreceptors

Kayla F. Titialii-Torres, Ann C. Morris

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Diabetes is linked to various long-term complications in adults, such as neuropathy, nephropathy and diabetic retinopathy. Diabetes poses additional risks for pregnant women, because glucose passes across the placenta, and excess maternal glucose can result in diabetic embryopathy. While many studies have examined the teratogenic effects of maternal diabetes on fetal heart development, little is known about the consequences of maternal hyperglycemia on the development of the embryonic retina. To address this question, we investigated retinal development in two models of embryonic hyperglycemia in zebrafish. Strikingly, we found that hyperglycemic larvae displayed a significant reduction in photoreceptors and horizontal cells, whereas other retinal neurons were not affected. We also observed reactive gliosis and abnormal optokinetic responses in hyperglycemic larvae. Further analysis revealed delayed retinal cell differentiation in hyperglycemic embryos that coincided with increased reactive oxygen species (ROS). Our results suggest that embryonic hyperglycemia causes abnormal retinal development via altered timing of cell differentiation and ROS production, which is accompanied by visual defects. Further studies using zebrafish models of hyperglycemia will allow us to understand the molecular mechanisms underlying these effects.

Original language	English
Article number	jcs259187
Journal	Journal of Cell Science
Volume	135
Issue number	1
DOIs	https://doi.org/10.1242/jcs.259187
State	Published - Jan 2022

Bibliographical note

Funding Information:
The authors would like to thank Evelyn Turnbaugh and Lucas Vieira Francisco for exceptional zebrafish care. We also thank Dr Hannah Henson for conceptual input and Dr Cagney Coomer for editorial input. This work was supported by grants from the NIH National Eye Institute (R01EY021769, to A.C.M.), the WUSTL Diabetes Research Center, an NIH-funded program (P30DK020579) supported by the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK; to A.C.M.), the National Science Foundation Bridge to the Doctorate Fellowship (NSF HRD 2004710, to K.F.T.-T.), the University of Kentucky Lyman T. Johnson graduate fellowship (to K.F.T.-T.), the UK Biology Merit Fellowship (to K.F.T.-T.), and a Gertrude F. Ribble Mini Grant (to K.F.T.-T.). Deposited in PMC for release after 12 months.

Funding Information:
This work was supported by grants from the NIH National Eye Institute (R01EY021769, to A.C.M.), the WUSTL Diabetes Research Center, an NIH-funded program (P30DK020579) supported by the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK; to A.C.M.), the National Science Foundation Bridge to the Doctorate Fellowship (NSF HRD 2004710, to K.F.T.-T.), the University of Kentucky Lyman T. Johnson graduate fellowship (to K.F.T.-T.), the UK Biology Merit Fellowship (to K.F.T.-T.), and a Gertrude F. Ribble Mini Grant (to K.F.T.-T.). Deposited in PMC for release after 12 months.

Publisher Copyright:
© 2022. Published by The Company of Biologists Ltd

Keywords

Diabetes
Hyperglycemia
Photoreceptors
Retina
Zebrafish

ASJC Scopus subject areas

Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1242/jcs.259187

Cite this

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title = "Embryonic hyperglycemia perturbs the development of specific retinal cell types, including photoreceptors",

abstract = "Diabetes is linked to various long-term complications in adults, such as neuropathy, nephropathy and diabetic retinopathy. Diabetes poses additional risks for pregnant women, because glucose passes across the placenta, and excess maternal glucose can result in diabetic embryopathy. While many studies have examined the teratogenic effects of maternal diabetes on fetal heart development, little is known about the consequences of maternal hyperglycemia on the development of the embryonic retina. To address this question, we investigated retinal development in two models of embryonic hyperglycemia in zebrafish. Strikingly, we found that hyperglycemic larvae displayed a significant reduction in photoreceptors and horizontal cells, whereas other retinal neurons were not affected. We also observed reactive gliosis and abnormal optokinetic responses in hyperglycemic larvae. Further analysis revealed delayed retinal cell differentiation in hyperglycemic embryos that coincided with increased reactive oxygen species (ROS). Our results suggest that embryonic hyperglycemia causes abnormal retinal development via altered timing of cell differentiation and ROS production, which is accompanied by visual defects. Further studies using zebrafish models of hyperglycemia will allow us to understand the molecular mechanisms underlying these effects.",

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note = "Funding Information: The authors would like to thank Evelyn Turnbaugh and Lucas Vieira Francisco for exceptional zebrafish care. We also thank Dr Hannah Henson for conceptual input and Dr Cagney Coomer for editorial input. This work was supported by grants from the NIH National Eye Institute (R01EY021769, to A.C.M.), the WUSTL Diabetes Research Center, an NIH-funded program (P30DK020579) supported by the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK; to A.C.M.), the National Science Foundation Bridge to the Doctorate Fellowship (NSF HRD 2004710, to K.F.T.-T.), the University of Kentucky Lyman T. Johnson graduate fellowship (to K.F.T.-T.), the UK Biology Merit Fellowship (to K.F.T.-T.), and a Gertrude F. Ribble Mini Grant (to K.F.T.-T.). Deposited in PMC for release after 12 months. Funding Information: This work was supported by grants from the NIH National Eye Institute (R01EY021769, to A.C.M.), the WUSTL Diabetes Research Center, an NIH-funded program (P30DK020579) supported by the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK; to A.C.M.), the National Science Foundation Bridge to the Doctorate Fellowship (NSF HRD 2004710, to K.F.T.-T.), the University of Kentucky Lyman T. Johnson graduate fellowship (to K.F.T.-T.), the UK Biology Merit Fellowship (to K.F.T.-T.), and a Gertrude F. Ribble Mini Grant (to K.F.T.-T.). Deposited in PMC for release after 12 months. Publisher Copyright: {\textcopyright} 2022. Published by The Company of Biologists Ltd",

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Embryonic hyperglycemia perturbs the development of specific retinal cell types, including photoreceptors

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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