Emodin-8-O-β-D-glucopyranoside, a natural hydroxyanthraquinone glycoside from plant, suppresses cancer cell proliferation via p21-CDKs-Rb axis

Yiqun Li, Kaiming Li, Yan Zhao, Yong Li, Dengke Li, Liangliang Shen, Qing Wang, Hsin Sheng Yang, Zhenxiao Sun

Research output: Contribution to journalArticlepeer-review

Abstract

Emodin-8-O-β-D-glucopyranoside (EG), a natural hydroxyanthraquinone glycoside from some traditional medicinal plants, has been demonstrated to have potential antitumor effects in our previous studies. Herein, we confirm that EG remains stable in the cell culture medium. It suppresses cell viability and proliferation and induces G1 cell cycle arrest in human colorectal cancer and neuroblastoma cells in vitro. EG inhibits tumor growth in human colorectal cancer cell HCT 116-bearing xenograft mice with low toxicity in the liver and kidney. The transcriptome analysis shows that the p53 signaling pathway is the most enriched cellular pathway and EG affects the proliferation of HCT 116 cells through modulating cell cycle related genes, such as CDKN1A and Cyclin-dependent kinases (CDKs). We demonstrate that the protein expression level of p21 was up-regulated, and CDK1/CDK2 were reduced significantly in both HCT 116 and SH-SY5Y cells after EG treatment. The switch from hypo- to hyper-phosphorylated Retinoblastoma (Rb), which is believed as a result of activated CDKs, was inhibited when cells were treated with EG. These findings indicate that EG suppresses cancer cell proliferation via p21-CDKs-Rb axis.

Original languageEnglish
Article number115909
JournalToxicology and Applied Pharmacology
Volume438
DOIs
StatePublished - Mar 1 2022

Bibliographical note

Funding Information:
We thank Dr. Tiangong Lu (Beijing University of Chinese Medicine) for her critical reading of the manuscript. This work was financially supported by a grant from the National Natural Science Foundation of China (Grant Number. 81473418 ).

Funding Information:
We thank Dr. Tiangong Lu (Beijing University of Chinese Medicine) for her critical reading of the manuscript. This work was financially supported by a grant from the National Natural Science Foundation of China (Grant Number. 81473418).

Publisher Copyright:
© 2022

Keywords

  • CDK1/2
  • Emodin-8-O-β-D-glucopyranoside
  • Rb phosphorylation
  • Tumor growth inhibition
  • p21

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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