Endogenous Glycoprotein GPM6a Is Involved in Neurite Outgrowth in Rat Dorsal Root Ganglion Neurons

Gabriela I. Aparicio, Antonella León, Rocío Gutiérrez Fuster, Baylen Ravenscraft, Paula V. Monje, Camila Scorticati

Research output: Contribution to journalArticlepeer-review


The peripheral nervous system (PNS) has a unique ability for self-repair. Dorsal root ganglion (DRG) neurons regulate the expression of different molecules, such as neurotrophins and their receptors, to promote axon regeneration after injury. However, the molecular players driving axonal regrowth need to be better defined. The membrane glycoprotein GPM6a has been described to contribute to neuronal development and structural plasticity in central-nervous-system neurons. Recent evidence indicates that GPM6a interacts with molecules from the PNS, although its role in DRG neurons remains unknown. Here, we characterized the expression of GPM6a in embryonic and adult DRGs by combining analysis of public RNA-seq datasets with immunochemical approaches utilizing cultures of rat DRG explants and dissociated neuronal cells. M6a was detected on the cell surfaces of DRG neurons throughout development. Moreover, GPM6a was required for DRG neurite elongation in vitro. In summary, we provide evidence on GPM6a being present in DRG neurons for the first time. Data from our functional experiments support the idea that GPM6a could contribute to axon regeneration in the PNS.

Original languageEnglish
Article number594
Issue number4
StatePublished - Apr 2023

Bibliographical note

Funding Information:
This research was funded by Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) BID-ANPCyT-PICT 2016-1223 and BID-ANPCyT-PICT 2019-01051 to C.S. G.I.A. received support from the National Council for Research of Argentina (CONICET) (2017-2022), the Fulbright, Bunge & Born, and Williams Foundations program 2020–2021, and the International Society for Neurochemistry—Committee for Aid and Education in Neurochemistry (ISN-CAEN). P.V.M., and G.I.A. received support from the Department of Neurosurgery from the University of Kentucky (UK) and the Indiana Spinal Cord and Brain Injury Research Fund from the Indiana State Department of Health (grants 33997 and 43547). The contents of this manuscript were solely the responsibility of the researchers and do not necessarily represent the official views of the funding agencies. A.L., R.G.F., and B.R. are Ph.D. students. C.S. is an independent researcher from CONICET.

Publisher Copyright:
© 2023 by the authors.


  • GPM6A
  • dorsal root ganglion neurons
  • glycoprotein
  • neuritogenesis
  • peripheral nervous system

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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