Endothelin-2 induces oviductal contraction via endothelin receptor subtype A in rats

Linah Al-Alem, Phillip J. Bridges, Wen Su, Ming C. Gong, Marc Iglarz, Che Myong Ko

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


Proper function of the oviduct is critical to reproductive success with regulated contraction and relaxation facilitating transportation of the germ cells to the site of fertilization. Endothelin-2 (EDN2) is a potent vasoconstrictor produced by granulosa cells of the preovulatory follicle at the time of ovulation; however, whether this gonadotropin surge-induced peptide played a role in facilitating germ cell transportation by inducing oviductal contraction was unknown. The objectives of these experiments were (1) to determine whether the endothelin receptor system was present in the oviduct, (2) to test the hypothesis that EDN2 induces oviductal contraction via a specific endothelin receptor subtype, (3) to determine, as a possible alternate source of the ligand, whether mRNA for EDN2 was expressed in cumulus-oocyte complexes (COCs) within the oviduct, and (4) to determine whether EDN2 could overcome prostaglandin E2 (PGE2)-induced oviductal relaxation. Microarray and real-time PCR analysis indicated that mRNA for both the endothelin receptor subtypes (ETA and ETB) was present in the oviduct, whereas immunohistochemical examination revealed that ETA protein was the dominant isoform, present in the luminal epithelial cells of the oviduct. Real-time PCR analysis demonstrated that mRNA for EDN2 was expressed in COCs after ovulation. Isometric tension analysis indicated that EDN2 was a potent oviductal constrictor and that the contractile effiect of EDN2 was mediated by the ETA and not the ETB receptor subtype. The oviductal contraction induced by EDN2 also reversed oviductal relaxation induced by PGE2. In summary, ETA receptor-specific EDN2-induced contraction as a facilitator of oviductal function suggests a novel pathway involved in germ cell transport and hence mammalian fertility.

Original languageEnglish
Pages (from-to)383-391
Number of pages9
JournalJournal of Endocrinology
Issue number3
StatePublished - Jun 2007

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology


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