Endothelin activation of reactive oxygen species mediates stress-induced pressor response in dahl salt-sensitive prehypertensive rats

Gerard D'Angelo, Analia S. Loria, David M. Pollock, Jennifer S. Pollock

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Experiments were designed to test the hypothesis that endothelin (ET) and/or reactive oxygen species contribute to the pressor response induced by acute air jet stress in normotensive Dahl salt-sensitive rats maintained on a normal salt diet (prehypertensive). Mean arterial pressure was chronically monitored by telemetry before and after 3-day treatment with the free radical scavenger 4-hydroxy-2,2,6,6-tetramethyl piperidinoxyl (Tempol) or ET receptor antagonists ABT-627 (ET A antagonist) or A-182086 (ET A/B antagonist) supplied in the drinking water. Rats were restrained and subjected to pulsatile air jet stress (3 minutes). Plasma samples at baseline and during acute stress were analyzed for 8-isoprostane (measure of reactive oxygen species production) and ET. Neither Tempol nor ET receptor antagonist treatment had an effect on baseline mean arterial pressure or plasma 8-isoprostane. The pressor response to acute stress was accompanied by significant increases in plasma 8-isoprostane and ET. Tempol significantly reduced both the total pressor response (area under the curve) and the stress-mediated increase in plasma 8-isoprostane; conversely, Tempol had no effect on the stress-induced increase in plasma ET. Combined ETA/B antagonism, but not selective ETA receptor blockade, similarly suppressed the pressor response to stress and stress-mediated rise in 8-isoprostane. Together these results indicate that reactive oxygen species contribute to the pressor response to acute air jet stress. Furthermore, the increase in reactive oxygen species occurs downstream of ETB receptor activation.

Original languageEnglish
Pages (from-to)282-289
Number of pages8
JournalHypertension
Volume56
Issue number2
DOIs
StatePublished - Aug 2010

Keywords

  • Dahl salt-sensitive rat
  • air jet stress
  • blood pressure
  • endothelin
  • reactive oxygen species

ASJC Scopus subject areas

  • Internal Medicine

Fingerprint

Dive into the research topics of 'Endothelin activation of reactive oxygen species mediates stress-induced pressor response in dahl salt-sensitive prehypertensive rats'. Together they form a unique fingerprint.

Cite this