Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment

Alison Schroer; Gaspard Pardon; Erica Castillo; Cheavar Blair; Beth Pruitt

doi:10.1016/j.pbiomolbio.2018.12.001

Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment

Alison Schroer, Gaspard Pardon, Erica Castillo, Cheavar Blair, Beth Pruitt

Physiology

Research output: Contribution to journal › Review article › peer-review

13 Scopus citations

Abstract

The study of human cardiomyopathies and the development and testing of new therapies has long been limited by the availability of appropriate in vitro model systems. Cardiomyocytes are highly specialized cells whose internal structure and contractile function are sensitive to the local microenvironment and the combination of mechanical and biochemical cues they receive. The complementary technologies of human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) and microphysiological systems (MPS) allow for precise control of the genetics and microenvironment of human cells in in vitro contexts. These combined systems also enable quantitative measurement of mechanical function and intracellular organization. This review describes relevant factors in the myocardium microenvironment that affect CM structure and mechanical function and demonstrates the application of several engineered microphysiological systems for studying development, disease, and drug discovery.

Original language	English
Pages (from-to)	3-15
Number of pages	13
Journal	Progress in Biophysics and Molecular Biology
Volume	144
DOIs	https://doi.org/10.1016/j.pbiomolbio.2018.12.001
State	Published - Jul 2019

Bibliographical note

Funding Information:
This work was supported by the American Heart Association [ AHA 17CSA33590101 ], the National Science Foundation [ NSF CMMI 1662431 ; GRFP], the National Academies of Sciences, Engineering, and Medicine [Ford Foundation Fellowship] and the National Institutes of Health [ NIH 1R21HL13099301 ; TIMBS T32 ] and SNSF Early Postdoc.Mobility fellowship grant: [ P2SKP2 _164954 ].

Funding Information:
This work was supported by the American Heart Association [AHA 17CSA33590101], the National Science Foundation [NSF CMMI 1662431; GRFP], the National Academies of Sciences, Engineering, and Medicine [Ford Foundation Fellowship] and the National Institutes of Health [NIH 1R21HL13099301; TIMBS T32] and SNSF Early Postdoc.Mobility fellowship grant: [P2SKP2_164954].

Publisher Copyright:
© 2018

Keywords

Cardiac mechanobiology
Drug discovery
Heart-on-a-chip
Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs)
In vitro cardiac model
Microphysiological systems (MPS)

ASJC Scopus subject areas

Biophysics
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.pbiomolbio.2018.12.001

Cite this

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title = "Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment",

abstract = "The study of human cardiomyopathies and the development and testing of new therapies has long been limited by the availability of appropriate in vitro model systems. Cardiomyocytes are highly specialized cells whose internal structure and contractile function are sensitive to the local microenvironment and the combination of mechanical and biochemical cues they receive. The complementary technologies of human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) and microphysiological systems (MPS) allow for precise control of the genetics and microenvironment of human cells in in vitro contexts. These combined systems also enable quantitative measurement of mechanical function and intracellular organization. This review describes relevant factors in the myocardium microenvironment that affect CM structure and mechanical function and demonstrates the application of several engineered microphysiological systems for studying development, disease, and drug discovery.",

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note = "Funding Information: This work was supported by the American Heart Association [ AHA 17CSA33590101 ], the National Science Foundation [ NSF CMMI 1662431 ; GRFP], the National Academies of Sciences, Engineering, and Medicine [Ford Foundation Fellowship] and the National Institutes of Health [ NIH 1R21HL13099301 ; TIMBS T32 ] and SNSF Early Postdoc.Mobility fellowship grant: [ P2SKP2 _164954 ]. Funding Information: This work was supported by the American Heart Association [AHA 17CSA33590101], the National Science Foundation [NSF CMMI 1662431; GRFP], the National Academies of Sciences, Engineering, and Medicine [Ford Foundation Fellowship] and the National Institutes of Health [NIH 1R21HL13099301; TIMBS T32] and SNSF Early Postdoc.Mobility fellowship grant: [P2SKP2_164954]. Publisher Copyright: {\textcopyright} 2018",

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AB - The study of human cardiomyopathies and the development and testing of new therapies has long been limited by the availability of appropriate in vitro model systems. Cardiomyocytes are highly specialized cells whose internal structure and contractile function are sensitive to the local microenvironment and the combination of mechanical and biochemical cues they receive. The complementary technologies of human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) and microphysiological systems (MPS) allow for precise control of the genetics and microenvironment of human cells in in vitro contexts. These combined systems also enable quantitative measurement of mechanical function and intracellular organization. This review describes relevant factors in the myocardium microenvironment that affect CM structure and mechanical function and demonstrates the application of several engineered microphysiological systems for studying development, disease, and drug discovery.

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Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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