Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment

Alison Schroer, Gaspard Pardon, Erica Castillo, Cheavar Blair, Beth Pruitt

Research output: Contribution to journalReview articlepeer-review

15 Scopus citations

Abstract

The study of human cardiomyopathies and the development and testing of new therapies has long been limited by the availability of appropriate in vitro model systems. Cardiomyocytes are highly specialized cells whose internal structure and contractile function are sensitive to the local microenvironment and the combination of mechanical and biochemical cues they receive. The complementary technologies of human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) and microphysiological systems (MPS) allow for precise control of the genetics and microenvironment of human cells in in vitro contexts. These combined systems also enable quantitative measurement of mechanical function and intracellular organization. This review describes relevant factors in the myocardium microenvironment that affect CM structure and mechanical function and demonstrates the application of several engineered microphysiological systems for studying development, disease, and drug discovery.

Original languageEnglish
Pages (from-to)3-15
Number of pages13
JournalProgress in Biophysics and Molecular Biology
Volume144
DOIs
StatePublished - Jul 2019

Bibliographical note

Publisher Copyright:
© 2018

Keywords

  • Cardiac mechanobiology
  • Drug discovery
  • Heart-on-a-chip
  • Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs)
  • In vitro cardiac model
  • Microphysiological systems (MPS)

ASJC Scopus subject areas

  • Biophysics
  • Molecular Biology

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