Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment

Alison Schroer; Gaspard Pardon; Erica Castillo; Cheavar Blair; Beth Pruitt

doi:10.1016/j.pbiomolbio.2018.12.001

Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment

Alison Schroer, Gaspard Pardon, Erica Castillo, Cheavar Blair, Beth Pruitt

Research output: Contribution to journal › Review article › peer-review

22 Scopus citations

Abstract

The study of human cardiomyopathies and the development and testing of new therapies has long been limited by the availability of appropriate in vitro model systems. Cardiomyocytes are highly specialized cells whose internal structure and contractile function are sensitive to the local microenvironment and the combination of mechanical and biochemical cues they receive. The complementary technologies of human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) and microphysiological systems (MPS) allow for precise control of the genetics and microenvironment of human cells in in vitro contexts. These combined systems also enable quantitative measurement of mechanical function and intracellular organization. This review describes relevant factors in the myocardium microenvironment that affect CM structure and mechanical function and demonstrates the application of several engineered microphysiological systems for studying development, disease, and drug discovery.

Original language	English
Pages (from-to)	3-15
Number of pages	13
Journal	Progress in Biophysics and Molecular Biology
Volume	144
DOIs	https://doi.org/10.1016/j.pbiomolbio.2018.12.001
State	Published - Jul 2019

Bibliographical note

Publisher Copyright:
© 2018

Funding

This work was supported by the American Heart Association [ AHA 17CSA33590101 ], the National Science Foundation [ NSF CMMI 1662431 ; GRFP], the National Academies of Sciences, Engineering, and Medicine [Ford Foundation Fellowship] and the National Institutes of Health [ NIH 1R21HL13099301 ; TIMBS T32 ] and SNSF Early Postdoc.Mobility fellowship grant: [ P2SKP2 _164954 ]. This work was supported by the American Heart Association [AHA 17CSA33590101], the National Science Foundation [NSF CMMI 1662431; GRFP], the National Academies of Sciences, Engineering, and Medicine [Ford Foundation Fellowship] and the National Institutes of Health [NIH 1R21HL13099301; TIMBS T32] and SNSF Early Postdoc.Mobility fellowship grant: [P2SKP2_164954].

Funders	Funder number
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China	CMMI 1662431
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China
National Institutes of Health (NIH)	TIMBS T32
National Institutes of Health (NIH)
Ford Foundation
National Heart, Lung, and Blood Institute Family Blood Pressure Program	R21HL130993
National Heart, Lung, and Blood Institute Family Blood Pressure Program
American the American Heart Association	17CSA33590101
American the American Heart Association
National Academies of Sciences, Engineering, and Medicine
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung	P2SKP2 _164954
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung

Keywords

Cardiac mechanobiology
Drug discovery
Heart-on-a-chip
Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs)
In vitro cardiac model
Microphysiological systems (MPS)

ASJC Scopus subject areas

Biophysics
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.pbiomolbio.2018.12.001

Cite this

@article{3b9bf684ca664d17a79654308f5443ee,

title = "Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment",

abstract = "The study of human cardiomyopathies and the development and testing of new therapies has long been limited by the availability of appropriate in vitro model systems. Cardiomyocytes are highly specialized cells whose internal structure and contractile function are sensitive to the local microenvironment and the combination of mechanical and biochemical cues they receive. The complementary technologies of human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) and microphysiological systems (MPS) allow for precise control of the genetics and microenvironment of human cells in in vitro contexts. These combined systems also enable quantitative measurement of mechanical function and intracellular organization. This review describes relevant factors in the myocardium microenvironment that affect CM structure and mechanical function and demonstrates the application of several engineered microphysiological systems for studying development, disease, and drug discovery.",

keywords = "Cardiac mechanobiology, Drug discovery, Heart-on-a-chip, Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs), In vitro cardiac model, Microphysiological systems (MPS)",

author = "Alison Schroer and Gaspard Pardon and Erica Castillo and Cheavar Blair and Beth Pruitt",

note = "Publisher Copyright: {\textcopyright} 2018",

year = "2019",

month = jul,

doi = "10.1016/j.pbiomolbio.2018.12.001",

language = "English",

volume = "144",

pages = "3--15",

journal = "Progress in Biophysics and Molecular Biology",

issn = "0079-6107",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment

AU - Schroer, Alison

AU - Pardon, Gaspard

AU - Castillo, Erica

AU - Blair, Cheavar

AU - Pruitt, Beth

PY - 2019/7

Y1 - 2019/7

N2 - The study of human cardiomyopathies and the development and testing of new therapies has long been limited by the availability of appropriate in vitro model systems. Cardiomyocytes are highly specialized cells whose internal structure and contractile function are sensitive to the local microenvironment and the combination of mechanical and biochemical cues they receive. The complementary technologies of human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) and microphysiological systems (MPS) allow for precise control of the genetics and microenvironment of human cells in in vitro contexts. These combined systems also enable quantitative measurement of mechanical function and intracellular organization. This review describes relevant factors in the myocardium microenvironment that affect CM structure and mechanical function and demonstrates the application of several engineered microphysiological systems for studying development, disease, and drug discovery.

AB - The study of human cardiomyopathies and the development and testing of new therapies has long been limited by the availability of appropriate in vitro model systems. Cardiomyocytes are highly specialized cells whose internal structure and contractile function are sensitive to the local microenvironment and the combination of mechanical and biochemical cues they receive. The complementary technologies of human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) and microphysiological systems (MPS) allow for precise control of the genetics and microenvironment of human cells in in vitro contexts. These combined systems also enable quantitative measurement of mechanical function and intracellular organization. This review describes relevant factors in the myocardium microenvironment that affect CM structure and mechanical function and demonstrates the application of several engineered microphysiological systems for studying development, disease, and drug discovery.

KW - Cardiac mechanobiology

KW - Drug discovery

KW - Heart-on-a-chip

KW - Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs)

KW - In vitro cardiac model

KW - Microphysiological systems (MPS)

UR - https://www.scopus.com/pages/publications/85067289174

UR - https://www.scopus.com/inward/citedby.url?scp=85067289174&partnerID=8YFLogxK

U2 - 10.1016/j.pbiomolbio.2018.12.001

DO - 10.1016/j.pbiomolbio.2018.12.001

M3 - Review article

C2 - 30579630

AN - SCOPUS:85067289174

SN - 0079-6107

VL - 144

SP - 3

EP - 15

JO - Progress in Biophysics and Molecular Biology

JF - Progress in Biophysics and Molecular Biology

ER -

Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this