Abstract
W/c-kit mutant mice accept engraftment by small numbers of normal hematopoietic stem cells without the necessity for myeloablation. One explanation for this observation is that a deficiency of Kit receptors reduces the number of primitive hematopoietic stem cells and increases the number of available 'niches' or 'space' in the marrow. As a test of this model, we transplanted a series of unirradiated W mutant mice with donor marrow cells of the identical mutant genotype. Despite the intrinsic anemia and hematopoietic defect of severely affected W(x)/W(v) and mildly affected W(v) months after transplantation with 1-4 x 107 marrow cells of the same W(x)/W(v) and W(v)/+ genotypes, respectively. In contrast, both genotypes were engrafted, as judged by sustained proliferation of donor-derived RBC, after a second transplant with equal numbers of +/+ cells. The inability of W(x)/W(v) marrow to proliferate in W(x)/W(v) hosts was not due to an absence of transplantable stem cells, however, as W(x)/W(v) cells were capable of sustained engraftment and proliferation in irradiated W(x)/W(v) recipients. We conclude that when donor and host are equivalent for Kit receptor function, W mutant mice do not accept marrow grafts more readily than wild-type mice. The results suggest that a deficiency of host Kit receptor function promotes engraftment of normal stem cells not by increasing marrow space, but by providing an advantage to donor cells in competition for marrow stroma or for self-renewal and differentiation.
Original language | English |
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Pages (from-to) | 209-213 |
Number of pages | 5 |
Journal | Experimental Hematology |
Volume | 24 |
Issue number | 2 |
State | Published - 1996 |
Keywords
- bone marrow transplantation
- hematopoietic stem cells
- proto-oncogene protein Kit
- stromal cells
ASJC Scopus subject areas
- Molecular Biology
- Hematology
- Genetics
- Cell Biology
- Cancer Research