Enhanced production of podophyllotoxin by Podophyllum hexandrum using in situ cell retention bioreactor

Saurabh Chattopadhyay, Virendra S. Bisaria, Ashok K. Srivastava

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The rhizomes of the rare plant Podophyllum hexandrum contain podophyllotoxin, which is a precursor of the anticancer drugs etoposide and teniposide. Batch cultivation of Podophyllum hexandrum was conducted using optimized medium in a 3 L bioreactor, which resulted in biomass and podophyllotoxin concentrations of 21.4 g/L and 13.8 mg/L in 24 and 26 days, respectively. The batch kinetics was used to identify the mathematical model. The model was extrapolated to identify the nutrient feeding rate (150 mL/d) and substrate concentration (105 g/L) in the incoming feed for nonlimiting and noninhibitory glucose concentration in the cell retention bioreactor. An improvement in cell growth to 53 g/L and intracellular podophyllotoxin accumulation of 48.8 mg/L was achieved in 60 days, when the bioreactor was operated in continuous cell retention cultivation mode.

Original languageEnglish
Pages (from-to)1026-1028
Number of pages3
JournalBiotechnology Progress
Volume19
Issue number3
DOIs
StatePublished - May 2003

ASJC Scopus subject areas

  • Biotechnology

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