TY - JOUR
T1 - Enhanced production of podophyllotoxin by Podophyllum hexandrum using in situ cell retention bioreactor
AU - Chattopadhyay, Saurabh
AU - Bisaria, Virendra S.
AU - Srivastava, Ashok K.
PY - 2003/5
Y1 - 2003/5
N2 - The rhizomes of the rare plant Podophyllum hexandrum contain podophyllotoxin, which is a precursor of the anticancer drugs etoposide and teniposide. Batch cultivation of Podophyllum hexandrum was conducted using optimized medium in a 3 L bioreactor, which resulted in biomass and podophyllotoxin concentrations of 21.4 g/L and 13.8 mg/L in 24 and 26 days, respectively. The batch kinetics was used to identify the mathematical model. The model was extrapolated to identify the nutrient feeding rate (150 mL/d) and substrate concentration (105 g/L) in the incoming feed for nonlimiting and noninhibitory glucose concentration in the cell retention bioreactor. An improvement in cell growth to 53 g/L and intracellular podophyllotoxin accumulation of 48.8 mg/L was achieved in 60 days, when the bioreactor was operated in continuous cell retention cultivation mode.
AB - The rhizomes of the rare plant Podophyllum hexandrum contain podophyllotoxin, which is a precursor of the anticancer drugs etoposide and teniposide. Batch cultivation of Podophyllum hexandrum was conducted using optimized medium in a 3 L bioreactor, which resulted in biomass and podophyllotoxin concentrations of 21.4 g/L and 13.8 mg/L in 24 and 26 days, respectively. The batch kinetics was used to identify the mathematical model. The model was extrapolated to identify the nutrient feeding rate (150 mL/d) and substrate concentration (105 g/L) in the incoming feed for nonlimiting and noninhibitory glucose concentration in the cell retention bioreactor. An improvement in cell growth to 53 g/L and intracellular podophyllotoxin accumulation of 48.8 mg/L was achieved in 60 days, when the bioreactor was operated in continuous cell retention cultivation mode.
UR - http://www.scopus.com/inward/record.url?scp=0038119624&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038119624&partnerID=8YFLogxK
U2 - 10.1021/bp025765k
DO - 10.1021/bp025765k
M3 - Article
C2 - 12790673
AN - SCOPUS:0038119624
SN - 8756-7938
VL - 19
SP - 1026
EP - 1028
JO - Biotechnology Progress
JF - Biotechnology Progress
IS - 3
ER -