TY - JOUR
T1 - Entrainment of the Circadian Clock of the Enteric Bacterium Klebsiella aerogenes by Temperature Cycles
AU - Paulose, Jiffin K.
AU - Cassone, Charles V.
AU - Graniczkowska, Kinga B.
AU - Cassone, Vincent M.
N1 - Publisher Copyright:
© 2019 The Author(s)
PY - 2019/9/27
Y1 - 2019/9/27
N2 - The gastrointestinal bacterium Klebsiella (née Enterobacter) aerogenes expresses an endogenously generated, temperature-compensated circadian rhythm in swarming motility. We hypothesized that this rhythm may be synchronized/entrained in vivo by body temperature (TB). To determine entrainment, cultures expressing bioluminescence were exposed to temperature cycles of 1°C (35°C–36°C) or 3°C (34°C–37°C) in amplitude at periods (T-cycles) of T = 22, T = 24, or T = 28 h. Bacteria entrained to all T-cycles at both amplitudes and with stable phase relationships. A high-amplitude phase response curve (PRC) in response to 1-h pulses of 3°C temperature spike (34°C–37°C) at different circadian phases was constructed, revealing a Type-0 phase resetting paradigm. Furthermore, real-time bioluminescence imaging revealed a spatiotemporal pattern to the circadian rhythm. These data are consistent with the hypothesis that the K. aerogenes circadian clock entrains to its host via detection of and phase shifting to the daily pattern of TB.
AB - The gastrointestinal bacterium Klebsiella (née Enterobacter) aerogenes expresses an endogenously generated, temperature-compensated circadian rhythm in swarming motility. We hypothesized that this rhythm may be synchronized/entrained in vivo by body temperature (TB). To determine entrainment, cultures expressing bioluminescence were exposed to temperature cycles of 1°C (35°C–36°C) or 3°C (34°C–37°C) in amplitude at periods (T-cycles) of T = 22, T = 24, or T = 28 h. Bacteria entrained to all T-cycles at both amplitudes and with stable phase relationships. A high-amplitude phase response curve (PRC) in response to 1-h pulses of 3°C temperature spike (34°C–37°C) at different circadian phases was constructed, revealing a Type-0 phase resetting paradigm. Furthermore, real-time bioluminescence imaging revealed a spatiotemporal pattern to the circadian rhythm. These data are consistent with the hypothesis that the K. aerogenes circadian clock entrains to its host via detection of and phase shifting to the daily pattern of TB.
KW - Biological Sciences
KW - Chronobiology
KW - Microbiology
UR - http://www.scopus.com/inward/record.url?scp=85072300816&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85072300816&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2019.09.007
DO - 10.1016/j.isci.2019.09.007
M3 - Article
AN - SCOPUS:85072300816
VL - 19
SP - 1202
EP - 1213
JO - iScience
JF - iScience
ER -